NB100-58842
antibody from Novus Biologicals
Targeting: NANOG
FLJ12581, FLJ40451
Antibody data
- Antibody Data
- Antigen structure
- References [44]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-58842 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58842, RRID:AB_2150116
- Product name
- Rabbit Polyclonal Nanog Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Metabolic switch and epithelial-mesenchymal transition cooperate to regulate pluripotency.
Alterations in Phosphorylation of Hepatocyte Ribosomal Protein S6 Control Plasmodium Liver Stage Infection.
Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells.
Direct conversion of mouse embryonic fibroblast to osteoblast cells using hLMP-3 with Yamanaka factors.
Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.
Generation of Rybp homozygous knockout murine ES cell line GIBHe001-A-1 by using CRISPR/Cas9 technology.
Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.
Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance.
Tousled-like kinase 1 is a negative regulator of core transcription factors in murine embryonic stem cells.
Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
miR‑146b‑5p promotes the neural conversion of pluripotent stem cells by targeting Smad4.
RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells.
Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells.
Characteristics and Properties of Mesenchymal Stem Cells Derived From Microfragmented Adipose Tissue.
Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts.
Chromosome transplantation as a novel approach for correcting complex genomic disorders.
The pluripotency factor NANOG promotes the formation of squamous cell carcinomas.
The double inhibition of endogenously produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells.
Prognostic value of stem cell quantification in stage II colon cancer.
TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells.
Reprogramming in vivo produces teratomas and iPS cells with totipotency features.
The positional identity of mouse ES cell-generated neurons is affected by BMP signaling.
n-Butylidenephthalide (BP) maintains stem cell pluripotency by activating Jak2/Stat3 pathway and increases the efficiency of iPS cells generation.
Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem cells.
The first reported generation of several induced pluripotent stem cell lines from homozygous and heterozygous Huntington's disease patients demonstrates mutation related enhanced lysosomal activity.
Induced pluripotent stem cell lines from Huntington's disease mice undergo neuronal differentiation while showing alterations in the lysosomal pathway.
Suv4-20h abrogation enhances telomere elongation during reprogramming and confers a higher tumorigenic potential to iPS cells.
Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.
NPR-A regulates self-renewal and pluripotency of embryonic stem cells.
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Low concentrations of nitric oxide delay the differentiation of embryonic stem cells and promote their survival.
Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice.
Phosphorylation stabilizes Nanog by promoting its interaction with Pin1.
The pluripotency-associated gene Dppa4 is dispensable for embryonic stem cell identity and germ cell development but essential for embryogenesis.
Smarcc1/Baf155 couples self-renewal gene repression with changes in chromatin structure in mouse embryonic stem cells.
Direct cell reprogramming is a stochastic process amenable to acceleration.
Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells.
Generation of multipotent cell lines from a distinct population of male germ line stem cells.
Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells.
Activated Kras, but not Hras or Nras, may initiate tumors of endodermal origin via stem cell expansion.
Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease.
Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells.
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog.
Sun H, Yang X, Liang L, Zhang M, Li Y, Chen J, Wang F, Yang T, Meng F, Lai X, Li C, He J, He M, Xu Q, Li Q, Lin L, Pei D, Zheng H
The EMBO journal 2020 Apr 15;39(8):e102961
The EMBO journal 2020 Apr 15;39(8):e102961
Alterations in Phosphorylation of Hepatocyte Ribosomal Protein S6 Control Plasmodium Liver Stage Infection.
Glennon EKK, Austin LS, Arang N, Kain HS, Mast FD, Vijayan K, Aitchison JD, Kappe SHI, Kaushansky A
Cell reports 2019 Mar 19;26(12):3391-3399.e4
Cell reports 2019 Mar 19;26(12):3391-3399.e4
Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells.
Castelli A, Susani L, Menale C, Muggeo S, Caldana E, Strina D, Cassani B, Recordati C, Scanziani E, Ficara F, Villa A, Vezzoni P, Paulis M
Stem cells (Dayton, Ohio) 2019 Jul;37(7):876-887
Stem cells (Dayton, Ohio) 2019 Jul;37(7):876-887
Direct conversion of mouse embryonic fibroblast to osteoblast cells using hLMP-3 with Yamanaka factors.
Ahmed MF, El-Sayed AK, Chen H, Zhao R, Jin K, Zuo Q, Zhang Y, Li B
The international journal of biochemistry & cell biology 2019 Jan;106:84-95
The international journal of biochemistry & cell biology 2019 Jan;106:84-95
Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.
Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K
Biochemical and biophysical research communications 2019 Feb 19;509(4):1008-1014
Biochemical and biophysical research communications 2019 Feb 19;509(4):1008-1014
Generation of Rybp homozygous knockout murine ES cell line GIBHe001-A-1 by using CRISPR/Cas9 technology.
Liu Z, Yao M, Yao H, Hu G, Qin B
Stem cell research 2019 Dec;41:101638
Stem cell research 2019 Dec;41:101638
Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.
Malik V, Glaser LV, Zimmer D, Velychko S, Weng M, Holzner M, Arend M, Chen Y, Srivastava Y, Veerapandian V, Shah Z, Esteban MA, Wang H, Chen J, Schöler HR, Hutchins AP, Meijsing SH, Pott S, Jauch R
Nature communications 2019 Aug 2;10(1):3477
Nature communications 2019 Aug 2;10(1):3477
Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance.
Liu C, Cai Z, Jin G, Peng D, Pan BS, Zhang X, Han F, Xu X, Lin HK
Cell discovery 2018;4:54
Cell discovery 2018;4:54
Tousled-like kinase 1 is a negative regulator of core transcription factors in murine embryonic stem cells.
Lee J, Kim MS, Park SH, Jang YK
Scientific reports 2018 Jan 10;8(1):334
Scientific reports 2018 Jan 10;8(1):334
Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
Veerapandian V, Ackermann JO, Srivastava Y, Malik V, Weng M, Yang X, Jauch R
Stem cell reports 2018 Aug 14;11(2):593-606
Stem cell reports 2018 Aug 14;11(2):593-606
miR‑146b‑5p promotes the neural conversion of pluripotent stem cells by targeting Smad4.
Zhang N, Lyu Y, Pan X, Xu L, Xuan A, He X, Huang W, Long D
International journal of molecular medicine 2017 Sep;40(3):814-824
International journal of molecular medicine 2017 Sep;40(3):814-824
RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells.
Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, Rocchiccioli S, D'Onofrio M, Cremisi F
Genome biology 2016 May 6;17(1):94
Genome biology 2016 May 6;17(1):94
Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells.
Kim J, Liu Y, Qiu M, Xu Y
Oncogene 2016 Mar 10;35(10):1334-40
Oncogene 2016 Mar 10;35(10):1334-40
Characteristics and Properties of Mesenchymal Stem Cells Derived From Microfragmented Adipose Tissue.
Carelli S, Messaggio F, Canazza A, Hebda DM, Caremoli F, Latorre E, Grimoldi MG, Colli M, Bulfamante G, Tremolada C, Di Giulio AM, Gorio A
Cell transplantation 2015;24(7):1233-52
Cell transplantation 2015;24(7):1233-52
Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts.
Neri T, Muggeo S, Paulis M, Caldana ME, Crisafulli L, Strina D, Focarelli ML, Faggioli F, Recordati C, Scaramuzza S, Scanziani E, Mantero S, Buracchi C, Sobacchi C, Lombardo A, Naldini L, Vezzoni P, Villa A, Ficara F
Stem cell reports 2015 Oct 13;5(4):558-68
Stem cell reports 2015 Oct 13;5(4):558-68
Chromosome transplantation as a novel approach for correcting complex genomic disorders.
Paulis M, Castelli A, Susani L, Lizier M, Lagutina I, Focarelli ML, Recordati C, Uva P, Faggioli F, Neri T, Scanziani E, Galli C, Lucchini F, Villa A, Vezzoni P
Oncotarget 2015 Nov 3;6(34):35218-30
Oncotarget 2015 Nov 3;6(34):35218-30
The pluripotency factor NANOG promotes the formation of squamous cell carcinomas.
Palla AR, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez-López G, Dominguez O, Dueñas M, Paramio JM, Serrano M
Scientific reports 2015 May 19;5:10205
Scientific reports 2015 May 19;5:10205
The double inhibition of endogenously produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells.
Bertacchi M, Pandolfini L, D'Onofrio M, Brandi R, Cremisi F
Developmental neurobiology 2015 Jan;75(1):66-79
Developmental neurobiology 2015 Jan;75(1):66-79
Prognostic value of stem cell quantification in stage II colon cancer.
Vaz MA, Martinez JC, Devesa JM, Trill JD, Abraira V, Riquelme A, Carrato A
PloS one 2014;9(2):e88480
PloS one 2014;9(2):e88480
TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells.
Schneider RP, Garrobo I, Foronda M, Palacios JA, Marión RM, Flores I, Ortega S, Blasco MA
Nature communications 2013;4:1946
Nature communications 2013;4:1946
Reprogramming in vivo produces teratomas and iPS cells with totipotency features.
Abad M, Mosteiro L, Pantoja C, Cañamero M, Rayon T, Ors I, Graña O, Megías D, Domínguez O, Martínez D, Manzanares M, Ortega S, Serrano M
Nature 2013 Oct 17;502(7471):340-5
Nature 2013 Oct 17;502(7471):340-5
The positional identity of mouse ES cell-generated neurons is affected by BMP signaling.
Bertacchi M, Pandolfini L, Murenu E, Viegi A, Capsoni S, Cellerino A, Messina A, Casarosa S, Cremisi F
Cellular and molecular life sciences : CMLS 2013 Mar;70(6):1095-111
Cellular and molecular life sciences : CMLS 2013 Mar;70(6):1095-111
n-Butylidenephthalide (BP) maintains stem cell pluripotency by activating Jak2/Stat3 pathway and increases the efficiency of iPS cells generation.
Liu SP, Harn HJ, Chien YJ, Chang CH, Hsu CY, Fu RH, Huang YC, Chen SY, Shyu WC, Lin SZ
PloS one 2012;7(9):e44024
PloS one 2012;7(9):e44024
Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem cells.
Deshpande AM, Khalid O, Kim JJ, Kim Y, Lindgren A, Clark AT, Wong DT
Stem cells and development 2012 Nov 1;21(16):3010-8
Stem cells and development 2012 Nov 1;21(16):3010-8
The first reported generation of several induced pluripotent stem cell lines from homozygous and heterozygous Huntington's disease patients demonstrates mutation related enhanced lysosomal activity.
Camnasio S, Delli Carri A, Lombardo A, Grad I, Mariotti C, Castucci A, Rozell B, Lo Riso P, Castiglioni V, Zuccato C, Rochon C, Takashima Y, Diaferia G, Biunno I, Gellera C, Jaconi M, Smith A, Hovatta O, Naldini L, Di Donato S, Feki A, Cattaneo E
Neurobiology of disease 2012 Apr;46(1):41-51
Neurobiology of disease 2012 Apr;46(1):41-51
Induced pluripotent stem cell lines from Huntington's disease mice undergo neuronal differentiation while showing alterations in the lysosomal pathway.
Castiglioni V, Onorati M, Rochon C, Cattaneo E
Neurobiology of disease 2012 Apr;46(1):30-40
Neurobiology of disease 2012 Apr;46(1):30-40
Suv4-20h abrogation enhances telomere elongation during reprogramming and confers a higher tumorigenic potential to iPS cells.
Marión RM, Schotta G, Ortega S, Blasco MA
PloS one 2011;6(10):e25680
PloS one 2011;6(10):e25680
Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.
Villasante A, Piazzolla D, Li H, Gomez-Lopez G, Djabali M, Serrano M
Cell cycle (Georgetown, Tex.) 2011 May 1;10(9):1488-98
Cell cycle (Georgetown, Tex.) 2011 May 1;10(9):1488-98
NPR-A regulates self-renewal and pluripotency of embryonic stem cells.
Abdelalim EM, Tooyama I
Cell death & disease 2011 Mar 10;2:e127
Cell death & disease 2011 Mar 10;2:e127
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Kohler EE, Cowan CE, Chatterjee I, Malik AB, Wary KK
Blood 2011 Feb 3;117(5):1761-9
Blood 2011 Feb 3;117(5):1761-9
Low concentrations of nitric oxide delay the differentiation of embryonic stem cells and promote their survival.
Tejedo JR, Tapia-Limonchi R, Mora-Castilla S, Cahuana GM, Hmadcha A, Martin F, Bedoya FJ, Soria B
Cell death & disease 2010 Oct 7;1:e80
Cell death & disease 2010 Oct 7;1:e80
Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice.
Shin J, Bossenz M, Chung Y, Ma H, Byron M, Taniguchi-Ishigaki N, Zhu X, Jiao B, Hall LL, Green MR, Jones SN, Hermans-Borgmeyer I, Lawrence JB, Bach I
Nature 2010 Oct 21;467(7318):977-81
Nature 2010 Oct 21;467(7318):977-81
Phosphorylation stabilizes Nanog by promoting its interaction with Pin1.
Moretto-Zita M, Jin H, Shen Z, Zhao T, Briggs SP, Xu Y
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 27;107(30):13312-7
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 27;107(30):13312-7
The pluripotency-associated gene Dppa4 is dispensable for embryonic stem cell identity and germ cell development but essential for embryogenesis.
Madan B, Madan V, Weber O, Tropel P, Blum C, Kieffer E, Viville S, Fehling HJ
Molecular and cellular biology 2009 Jun;29(11):3186-203
Molecular and cellular biology 2009 Jun;29(11):3186-203
Smarcc1/Baf155 couples self-renewal gene repression with changes in chromatin structure in mouse embryonic stem cells.
Schaniel C, Ang YS, Ratnakumar K, Cormier C, James T, Bernstein E, Lemischka IR, Paddison PJ
Stem cells (Dayton, Ohio) 2009 Dec;27(12):2979-91
Stem cells (Dayton, Ohio) 2009 Dec;27(12):2979-91
Direct cell reprogramming is a stochastic process amenable to acceleration.
Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van Oudenaarden A, Jaenisch R
Nature 2009 Dec 3;462(7273):595-601
Nature 2009 Dec 3;462(7273):595-601
Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells.
Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA
Genes & development 2008 Mar 15;22(6):746-55
Genes & development 2008 Mar 15;22(6):746-55
Generation of multipotent cell lines from a distinct population of male germ line stem cells.
Izadyar F, Pau F, Marh J, Slepko N, Wang T, Gonzalez R, Ramos T, Howerton K, Sayre C, Silva F
Reproduction (Cambridge, England) 2008 Jun;135(6):771-84
Reproduction (Cambridge, England) 2008 Jun;135(6):771-84
Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells.
Liang J, Wan M, Zhang Y, Gu P, Xin H, Jung SY, Qin J, Wong J, Cooney AJ, Liu D, Songyang Z
Nature cell biology 2008 Jun;10(6):731-9
Nature cell biology 2008 Jun;10(6):731-9
Activated Kras, but not Hras or Nras, may initiate tumors of endodermal origin via stem cell expansion.
Quinlan MP, Quatela SE, Philips MR, Settleman J
Molecular and cellular biology 2008 Apr;28(8):2659-74
Molecular and cellular biology 2008 Apr;28(8):2659-74
Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease.
Wernig M, Zhao JP, Pruszak J, Hedlund E, Fu D, Soldner F, Broccoli V, Constantine-Paton M, Isacson O, Jaenisch R
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 15;105(15):5856-61
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 15;105(15):5856-61
Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells.
Meissner A, Wernig M, Jaenisch R
Nature biotechnology 2007 Oct;25(10):1177-81
Nature biotechnology 2007 Oct;25(10):1177-81
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K, Bernstein BE, Jaenisch R
Nature 2007 Jul 19;448(7151):318-24
Nature 2007 Jul 19;448(7151):318-24
Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog.
Hough SR, Clements I, Welch PJ, Wiederholt KA
Stem cells (Dayton, Ohio) 2006 Jun;24(6):1467-75
Stem cells (Dayton, Ohio) 2006 Jun;24(6):1467-75
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-58842] - Mouse Nanog.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nanog Antibody [NB100-58842] - Differentiation of wt and Suv4-20dn teratomas. Representative images of Nanog staining in wt and Suv4-20dn teratomas. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0025680) licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep10205), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Immunohistochemistry (IHC) for NANOG of paraffin-embedded sections of tail skin from CTR and TG mice treated as indicated in (B). Two magnifications are shown for each tissue (bars correspond to 50 um). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep10205), licensed under a CC-BY licence.