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Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-13078 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Nanog (Ser71) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of SY5Y cells using a Phospho-Nanog pSer71 polyclonal antibody (Product # PA5-13078). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with a Phospho-Nanog pSer71 polyclonal antibody (Product # PA5-13078) (1:500, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Cytoplasmic actin was counterstained with red dye conjugated phalloidin (5.25 uM, 25 min).
