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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- AP22771PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22771PU-N, RRID:AB_10842786
- Product name
- anti NANOG
- Antibody type
- Polyclonal
- Antigen
- NANOG antibody was raised against kLH conjugated synthetic peptide selected from the center region of human NANOG.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 µg
- Concentration
- 0,25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT)Western blot analysis of NANOG in Hela cell line lysates (35 µg/lane). (RIGHT)Western blot analysis of NANOG in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NANOG in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Spleen (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22771PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Testis (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22771PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
