MA1-017-HRP

antibody from Invitrogen Antibodies

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-017-HRP

Provider

Invitrogen Antibodies

Product name

Nanog Monoclonal Antibody (23D2-3C6), HRP

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Western blot analysis of MA1-017-HRP detects an ~38 kDa protein in embryonal carcinoma cells. MA1-017-HRP shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

Reactivity

Human

Host

Mouse

Conjugate

Horseradish Peroxidase

Isotype

IgG

Antibody clone number

23D2-3C6

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nanog was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Nanog monoclonal antibody (Product # MA1-017-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Conjugate

Horseradish Peroxidase