Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-017-HRP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nanog Monoclonal Antibody (23D2-3C6), HRP
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Western blot analysis of MA1-017-HRP detects an ~38 kDa protein in embryonal carcinoma cells. MA1-017-HRP shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Antibody clone number
- 23D2-3C6
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Nanog was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Nanog monoclonal antibody (Product # MA1-017-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
- Conjugate
- Horseradish Peroxidase