Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324577 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NANOG Antibody (S285)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NANOG Antibody (S285)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NANOG
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.31 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of NANOG (arrow) using rabbit NANOG Antibody (S285) (Cat.# TA324577). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of NANOG Antibody (S285) (Cat.# TA324577) in HepG2 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with TA324577 NANOG (S285) antibody. SY5Y cells were incubated with TA324577 NANOG (S285) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
- Validation comment
- IF
