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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AF2729 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Nanog Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q80Z64- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming.
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse.
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch.
Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation.
Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
FBXL10 protects Polycomb-bound genes from hypermethylation.
Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
Small RNA-mediated regulation of iPS cell generation.
LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
Klf5 is involved in self-renewal of mouse embryonic stem cells.
He S, Wang F, Zhang Y, Chen J, Liang L, Li Y, Zhang M, Yang X, Pang H, Li Y, Huang X, Qin D, Pei D, Sun H, Zheng H
Cell discovery 2019;5:11
Cell discovery 2019;5:11
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA
Cell stem cell 2018 Apr 5;22(4):543-558.e12
Cell stem cell 2018 Apr 5;22(4):543-558.e12
Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse.
Miao YL, Gambini A, Zhang Y, Padilla-Banks E, Jefferson WN, Bernhardt ML, Huang W, Li L, Williams CJ
Biology of reproduction 2018 Apr 1;98(4):449-464
Biology of reproduction 2018 Apr 1;98(4):449-464
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Wang P, Wan WW, Xiong SL, Feng H, Wu N
Cell death discovery 2017;3:16105
Cell death discovery 2017;3:16105
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Maldonado M, Luu RJ, Ramos ME, Nam J
Stem cell research 2016 Sep;17(2):222-227
Stem cell research 2016 Sep;17(2):222-227
Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch.
Sun H, Liang L, Li Y, Feng C, Li L, Zhang Y, He S, Pei D, Guo Y, Zheng H
Scientific reports 2016 Aug 2;6:30903
Scientific reports 2016 Aug 2;6:30903
Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation.
Friend K, Brooks HA, Propson NE, Thomson JA, Kimble J
PloS one 2015;10(9):e0139076
PloS one 2015;10(9):e0139076
Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T
The Journal of biological chemistry 2015 Sep 11;290(37):22782-94
The Journal of biological chemistry 2015 Sep 11;290(37):22782-94
FBXL10 protects Polycomb-bound genes from hypermethylation.
Boulard M, Edwards JR, Bestor TH
Nature genetics 2015 May;47(5):479-85
Nature genetics 2015 May;47(5):479-85
Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
Kranc KR, Oliveira DV, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Bragança J
Stem cells (Dayton, Ohio) 2015 Mar;33(3):699-712
Stem cells (Dayton, Ohio) 2015 Mar;33(3):699-712
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
Stem cells and development 2015 Mar 1;24(5):597-609
Stem cells and development 2015 Mar 1;24(5):597-609
STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
Huang G, Yan H, Ye S, Tong C, Ying QL
Stem cells (Dayton, Ohio) 2014 May;32(5):1149-60
Stem cells (Dayton, Ohio) 2014 May;32(5):1149-60
The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
Yang W, Lee YH, Jones AE, Woolnough JL, Zhou D, Dai Q, Wu Q, Giles KE, Townes TM, Wang H
Nature communications 2014 May 2;5:3818
Nature communications 2014 May 2;5:3818
Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
Varga E, Nemes C, Davis RP, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity MK, Dinnyes A
Experimental cell research 2014 Apr 1;322(2):335-44
Experimental cell research 2014 Apr 1;322(2):335-44
Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
Tai CI, Ying QL
Journal of cell science 2013 Mar 1;126(Pt 5):1093-8
Journal of cell science 2013 Mar 1;126(Pt 5):1093-8
Small RNA-mediated regulation of iPS cell generation.
Li Z, Yang CS, Nakashima K, Rana TM
The EMBO journal 2011 Mar 2;30(5):823-34
The EMBO journal 2011 Mar 2;30(5):823-34
LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Göttgens B
Nature cell biology 2011 Jan;13(1):13-21
Nature cell biology 2011 Jan;13(1):13-21
Klf5 is involved in self-renewal of mouse embryonic stem cells.
Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T
Journal of cell science 2008 Aug 15;121(Pt 16):2629-34
Journal of cell science 2008 Aug 15;121(Pt 16):2629-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
