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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- IC2729G - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Nanog Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Green dye
- Antigen sequence
Q80Z64- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nanog in D3 Mouse Embryonic Stem Cells. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line on irradiated mouse embryonic fibroblasts using Goat Anti-Mouse Nanog Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC2729G) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line either untreated (dark orange filled histogram) or treated with retinoic acid for 3 days (light orange filled histogram) was stained with Goat Anti-Mouse Nanog Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2729G) or isotype control antibody (Catalog # IC108G, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
