LS-C359267

antibody from LSBio

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C359267

Provider

LSBio

Product name

NANOG Antibody (aa20-166, clone 1E6C4) LS-C359267

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1E6C4

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: Nanog Antibody (1E6C4) - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: Nanog Antibody (1E6C4) - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: Nanog Antibody (1E6C4) - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).