ABIN2508894

antibody from antibodies-online

Targeting: NANOG FLJ12581, FLJ40451

Antibody data

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Validation data

Product number: ABIN2508894 - Provider product page
Provider: antibodies-online
Product name: anti-Nanog Homeobox (NANOG) antibody (Biotin)
Antibody type: Polyclonal
Antigen: Other
Description: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Anti-Human Nanog specific antibody was purified by affinity chromatography and then biotinylated.
Reactivity: Human
Host: Rabbit
Conjugate: Biotin
Vial size: 50 μg
Storage: -20°C

NANOG protein structure - ABIN2508894 shown in red.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (ABIN2511049), (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen. Western blot analysis using Anti-proBDNF antibody (ABIN2511049), (1:400): 1. proBDNF (WT-human) (B-257), (20 ng). 2. proNGF (WT-human) (N-280), (200 ng). 3. recombinant proNT-3 (200 ng). 4. hBDNF (B-250), (200 ng). 5. mNGF 2.5S (Grade II) (N-100), (200 ng). 6. hNT-3 (N-260), (200 ng). Expression of proBDNF in rat cerebellum Immunohistochemical staining of rat cerebellum with Anti-proBDNF antibody (ABIN2511049). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (ABIN2511049), (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen. Western blot analysis using Anti-proBDNF antibody (ABIN2511049), (1:400): 1. proBDNF (WT-human) (B-257), (20 ng). 2. proNGF (WT-human) (N-280), (200 ng). 3. recombinant proNT-3 (200 ng). 4. hBDNF (B-250), (200 ng). 5. mNGF 2.5S (Grade II) (N-100), (200 ng). 6. hNT-3 (N-260), (200 ng). Expression of proBDNF in rat cerebellum Immunohistochemical staining of rat cerebellum with Anti-proBDNF antibody (ABIN2511049). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.