H00079923-M04

antibody from Abnova Corporation

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00079923-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00079923-M04, RRID:AB_534950

Product name

NANOG monoclonal antibody (M04), clone 3A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NANOG.

Antigen sequence

TRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNIL
NLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQ
KASAPTYPSLYSSYHQGCLVNPTGNLPM


Isotype

IgG

Antibody clone number

3A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NANOG monoclonal antibody (M04), clone 3A12. Western Blot analysis of NANOG expression in MCF-7.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NANOG is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol