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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2042 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2042, RRID:AB_1678580
- Product name
- NANOG monoclonal antibody, clone 5A10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NANOG.
- Isotype
- IgG
- Antibody clone number
- 5A10
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation.
Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma.
Differential regulation of the Oct-3/4 gene in cell culture model systems that parallel different stages of mammalian development.
Tay Y, Zhang J, Thomson AM, Lim B, Rigoutsos I
Nature 2008 Oct 23;455(7216):1124-8
Nature 2008 Oct 23;455(7216):1124-8
Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma.
Chiou SH, Yu CC, Huang CY, Lin SC, Liu CJ, Tsai TH, Chou SH, Chien CS, Ku HH, Lo JF
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4085-95
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4085-95
Differential regulation of the Oct-3/4 gene in cell culture model systems that parallel different stages of mammalian development.
Mallanna SK, Boer B, Desler M, Rizzino A
Molecular reproduction and development 2008 Aug;75(8):1247-57
Molecular reproduction and development 2008 Aug;75(8):1247-57
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- Experimental details
- The cell lysate of NIH/3T3 (35 ug) was resolved by SDS-PAGE and probed with NANOG monoclonal antibody, clone 5A10 (Cat # MAB2042) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
