Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-59737 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59737, RRID:AB_892306
- Product name
- Goat Polyclonal Nanog Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer.
Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H
Genes to cells : devoted to molecular & cellular mechanisms 2006 Sep;11(9):1115-23
Genes to cells : devoted to molecular & cellular mechanisms 2006 Sep;11(9):1115-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-59737] - Analysis of Nanog (C Terminus) using NB100-59737 at 0.05ug/ml in HeLa (nuclear) cell lysate (35ug protein in RIPA buffer).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Nanog Antibody [NB100-59737] - (5ug/ml) as the reporter with the capture rabbit antibody (5ug/ml).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nanog Antibody [NB100-59737] - IHC staining of paraffin embedded Human Testis using NB100-59737 at 2ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.