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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-18678 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nanog Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been successfully tested in a Sandwhich ELISA as both a capture and a detection antibody in combination with PA5-18406.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of nuclear HeLa cell lysate using Product # PA5-18678 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of nuclear HeLa cell lysate using Product # PA5-18678 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of Nanog using two Nanog antibodies. The reporter antibody (Product # PA5-18678) was used at a concentration of 5 µg/ml and the reporter antibody was used at a concentration of 5 µg/ml.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Testis using Product # PA5-18678 at a concentration of 2 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
