RQ6052

antibody from NSJ Bioreagents

Targeting: FAS APO-1, APT1, CD95, FAS1, TNFRSF6

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6052

Provider

NSJ Bioreagents

Product name

Fas Antibody

Antibody type

Polyclonal

Description

This highly specific Fas antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Fas antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat thymus and 2) mouse RAW264.7 cell lysate with Fas antibody. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse spleen cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse RAW264.7 cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.