PA5-34800

antibody from Invitrogen Antibodies

Targeting: BCL10 c-E10, CARMEN, CIPER, CLAP, mE10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34800

Provider

Invitrogen Antibodies

Product name

Bcl-10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, Raji, Neuro2A, GL261, C8D30. Predicted reactivity: Mouse (91%), Rat (92%), Pig (92%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.12 % SDS-PAGE. Bcl-10 Polyclonal Antibody (Product # PA5-34800) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bcl-10 Polyclonal Antibody (Product # PA5-34800). Sample (30 µg of whole cell lysate). Lane A: Raji. 12% SDS PAGE. Bcl-10 Polyclonal Antibody (Product # PA5-34800) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BCL10 protein stained by Bcl-10 Polyclonal Antibody (Product # PA5-34800) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BCL10 protein stained by Bcl-10 Polyclonal Antibody (Product # PA5-34800) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract. A. 40 µg Raji whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of BCL10 protein by 4 µg Bcl-10 Polyclonal Antibody (Product # PA5-34800). 12 % SDS-PAGE. The immunoprecipitated BCL10 protein was detected by Bcl-10 Polyclonal Antibody (Product # PA5-34800) diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Bcl-10 Polyclonal Antibody (Product # PA5-34800) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Bcl-10 Polyclonal Antibody (Product # PA5-34800) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract. A. 40 µg Raji whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of BCL10 protein by 4 µg Bcl-10 Polyclonal Antibody (Product # PA5-34800). 12 % SDS-PAGE. The immunoprecipitated BCL10 protein was detected by Bcl-10 Polyclonal Antibody (Product # PA5-34800) diluted at 1:500.