PA5-28946

antibody from Invitrogen Antibodies

Targeting: BCL10 c-E10, CARMEN, CIPER, CLAP, mE10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28946

Provider

Invitrogen Antibodies

Product name

Bcl-10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3.

Concentration

0.45 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bcl-10 Polyclonal Antibody (Product # PA5-28946). Sample (30 µg whole cell lysate). A: Hep G2. B: MOLT4. C: Raji. 12% SDS PAGE. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bcl-10 Polyclonal Antibody (Product # PA5-28946). Sample (30 µg of whole cell lysate). A: NIH-3T3. 12% SDS PAGE. Bcl-10 Polyclonal Antibody. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/mL for 30 min) and 5 µg of either control rabbit IgG or Bcl-10 Polyclonal Antibody (Product # PA5-28946). The precipitated DNA was detected by PCR with primer set targeting to IκBα.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of BCL10 protein by 4 µg Bcl-10 Polyclonal Antibody (Product # PA5-28946). 12 % SDS-PAGE. The immunoprecipitated BCL10 protein was detected by Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500.