PA5-28946

antibody from Invitrogen Antibodies

Targeting: BCL10 c-E10, CARMEN, CIPER, CLAP, mE10

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28946

Provider

Invitrogen Antibodies

Product name

Bcl-10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3. Predicted reactivity: Mouse (90%), Rat (91%), Pig (92%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.45 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bcl-10 Polyclonal Antibody (Product # PA5-28946). Sample (30 µg whole cell lysate). A: Hep G2. B: MOLT4. C: Raji. 12% SDS PAGE. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bcl-10 Polyclonal Antibody (Product # PA5-28946). Sample (30 µg of whole cell lysate). A: NIH-3T3. 12% SDS PAGE. Bcl-10 Polyclonal Antibody. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/mL for 30 min) and 5 µg of either control rabbit IgG or Bcl-10 Polyclonal Antibody (Product # PA5-28946). The precipitated DNA was detected by PCR with primer set targeting to IκBα.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl-10 Polyclonal Antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of BCL10 protein by 4 µg Bcl-10 Polyclonal Antibody (Product # PA5-28946). 12 % SDS-PAGE. The immunoprecipitated BCL10 protein was detected by Bcl-10 Polyclonal Antibody (Product # PA5-28946) diluted at 1:500.