Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 659901 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#659901, RRID:AB_2563399
- Product name
- Purified anti-BRCA1
- Antibody type
- Polyclonal
- Antigen
- Peptide mapping to the carboxy terminus of human BRCA1.
- Description
- BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, and pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase, while phosphorylated BRCA1 resides within inner chromosomal structures, centrosomes, and the cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein. It participates in transcription-coupled repair of oxidative DNA damage, and X-chromosome inactivation. BRCA1 can also function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination, and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- Poly26599
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of PBMC (lane 1) and A431 (lane 2) using anti-BRCA1 antibody (Poly26599). β actin antibody (Poly6221) was used as a loading control.
- Conjugate
- Unconjugated