659901

antibody from BioLegend
Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53
Provider product page for 659901
Western blot
Immunoprecipitation
Immunohistochemistry

Antibody data

Product number
659901 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#659901, RRID:AB_2563399
Product name
Purified anti-BRCA1
Antibody type
Polyclonal
Antigen
Peptide mapping to the carboxy terminus of human BRCA1.
Description
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms.  BRCA1 localizes to the mitotic spindle microtubules, centriole walls, and pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase, while phosphorylated BRCA1 resides within inner chromosomal structures, centrosomes, and the cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein. It participates in transcription-coupled repair of oxidative DNA damage, and X-chromosome inactivation. BRCA1 can also function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination, and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Conjugate
Unconjugated
Antibody clone number
Poly26599
Vial size
50 µl
Concentration
1.0 mg/ml
Storage
2°C-8°C
Handling
Ambient RT
BRCA1 protein structure - 659901 shown in red.