NBP1-41185

antibody from Novus Biologicals

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-41185

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-41185, RRID:AB_10005035

Product name

Mouse Monoclonal BRCA1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

BRCA immunohistochemistry for screening of BRCA mutation in epithelial ovarian cancer patients.

Manchana T, Tantbirojn P, Pohthipornthawat N
Gynecologic oncology reports 2020 Aug;33:100582

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Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?

Bosviel R, Dumollard E, Déchelotte P, Bignon YJ, Bernard-Gallon D
Omics : a journal of integrative biology 2012 May;16(5):235-44

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Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.

Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, Boiteux JP, Fontana L, Bignon YJ, Guy L, Bernard-Gallon DJ
In vivo (Athens, Greece) 2010 Jul-Aug;24(4):393-400

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An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.

Oishi H, Kitagawa H, Wada O, Takezawa S, Tora L, Kouzu-Fujita M, Takada I, Yano T, Yanagisawa J, Kato S
The Journal of biological chemistry 2006 Jan 6;281(1):20-6

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Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.

Bernard-Gallon DJ, Dechelotte PJ, Le Corre L, Chalabi N, Vissac-Sabatier C, Bignon YJ
Anticancer research 2004 Jan-Feb;24(1):321-4

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BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.

Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW
Oncogene 2003 Jun 12;22(24):3749-58

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BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.

Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5

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BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.

Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5

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Tumor suppressor p53 is required to modulate BRCA1 expression.

Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, Aaronson SA, Lee SW
Molecular and cellular biology 2000 Oct;20(20):7450-9

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Tumor suppressor p53 is required to modulate BRCA1 expression.

Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, Aaronson SA, Lee SW
Molecular and cellular biology 2000 Oct;20(20):7450-9

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Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms.

Lu M, Arrick BA
Oncogene 2000 Dec 14;19(54):6351-60

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ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation.

de Toledo SM, Azzam EI, Dahlberg WK, Gooding TB, Little JB
Oncogene 2000 Dec 14;19(54):6185-93

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.