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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- LS-C150139 - Provider product page
- Provider
- LSBio
- Product name
- Anti-PRKCE / PKC-Epsilon Antibody (aa580-737) LS-C150139
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 25µg
- Storage
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- PKC epsilon in Human Lung Cancer Tissue. PKC epsilon was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.
