LS-C376413

antibody from LSBio

Targeting: PRKCE

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C376413

Provider

LSBio

Product name

PRKCE / PKC-Epsilon Antibody (aa488-737) LS-C376413

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PRKCE in MCF-7 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of PRKCE Antibody in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: PRKCE Antibody (4µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (20µg)