TA502438

antibody from OriGene

Targeting: PRKCE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502438

Provider

OriGene

Proper citation

OriGene Cat#TA502438, RRID:AB_11125031

Product name

PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Antibody type

Monoclonal

Description

PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PRKCE

Isotype

IgG

Antibody clone number

OTI2E9

Vial size

100 µl

Concentration

0.46 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PRKCE mouse monoclonal antibody (TA502438) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE(RC217702).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC217702 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKCE antibody(TA502438), and then analyzed by flow cytometry.

Validation comment

FC