MA5-14908

antibody from Invitrogen Antibodies

Targeting: PRKCE

Antibody data

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Validation data

PRKCE protein structure - MA5-14908 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PKC epsilon was achieved by transfecting A549 with PKC epsilon specific siRNAs (Silencer® select Product # s11102, s11101). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKC epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Absence of signal upon siRNA mediated knock down confirms that antibody is specific to PKC epsilon.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6), SH-SY5Y (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with anti-PKC epsilon Antibody (Product # MA5-14908, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to PKC epsilon was observed across all the cell lines and tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC Epsilon in extracts from mouse brain and PC12 cells using PKC- Epsilon monoclonal antibody (Product # MA5-14908).