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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-54900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CASK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AYKIHLPETV EQLRKFNARR KLKGAVLAAV SSHKFNSFYG DPPEELPDFS EDPTSSGAVS QVLDSLEEIH ALTDCSEKDL DFLHSVFQDQ HLHTLLDLYD KINTKSSPQI RNPPSDAVQR AKEVLEEISC YPENNDAKE Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 96%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CASK in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a CASK Polyclonal Antibody (Product # PA5-54900).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CASK in human duodenum tissue shows strong cytoplasmic and membranous positivity. Samples were probed using a CASK Polyclonal Antibody (Product # PA5-54900).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CASK in human duodenum and testis tissues using CASK Polyclonal Antibody (Product # PA5-54900). Corresponding CASK RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CASK in human duodenum using CASK Polyclonal Antibody (Product # PA5-54900) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CASK in human testis using CASK Polyclonal Antibody (Product # PA5-54900) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CASK in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a CASK Polyclonal Antibody (Product # PA5-54900).
