MAB25031

antibody from Novus Biologicals

Targeting: CFTR ABC35, ABCC7, CF, CFTR/MRP, dJ760C5.1, MRP7, TNR-CFTR

Provider product page for MAB25031

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [41]
Comments [0]
Validations
Immunohistochemistry [2]

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Product number: MAB25031 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal CFTR Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Vial size: 50 ug
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

CFTR protein structure - MAB25031 shown in red.

Submitted references Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation.

Ortiz-Muñoz G, Yu MA, Lefrançais E, Mallavia B, Valet C, Tian JJ, Ranucci S, Wang KM, Liu Z, Kwaan N, Dawson D, Kleinhenz ME, Khasawneh FT, Haggie PM, Verkman AS, Looney MR
The Journal of clinical investigation 2020 Apr 1;130(4):2041-2053

Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation.

Malakpour Kolbadinezhad S, Coimbra J, Wilson JM
PloS one 2018;13(10):e0206206

Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene.

De Rocco D, Pompili B, Castellani S, Morini E, Cavinato L, Cimino G, Mariggiò MA, Guarnieri S, Conese M, Del Porto P, Ascenzioni F
International journal of molecular sciences 2018 Apr 17;19(4)

Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages.

Shrestha CL, Assani KD, Rinehardt H, Albastroiu F, Zhang S, Shell R, Amer AO, Schlesinger LS, Kopp BT
PloS one 2017;12(10):e0186169

Synergy of cAMP and calcium signaling pathways in CFTR regulation.

Bozoky Z, Ahmadi S, Milman T, Kim TH, Du K, Di Paola M, Pasyk S, Pekhletski R, Keller JP, Bear CE, Forman-Kay JD
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 14;114(11):E2086-E2095

Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR.

Figueira MF, Castiglione RC, de Lemos Barbosa CM, Ornellas FM, da Silva Feltran G, Morales MM, da Fonseca RN, de Souza-Menezes J
Physiological reports 2017 Jul;5(13)

Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling.

Schögler A, Blank F, Brügger M, Beyeler S, Tschanz SA, Regamey N, Casaulta C, Geiser T, Alves MP
Respiratory research 2017 Dec 28;18(1):215

Ammonia exposure affects the mRNA and protein expression levels of certain Rhesus glycoproteins in the gills of climbing perch.

Chen XL, Zhang B, Chng YR, Ong JLY, Chew SF, Wong WP, Lam SH, Nakada T, Ip YK
The Journal of experimental biology 2017 Aug 15;220(Pt 16):2916-2931

New use for an old drug: COX-independent anti-inflammatory effects of sulindac in models of cystic fibrosis.

Rocca J, Manin S, Hulin A, Aissat A, Verbecq-Morlot W, Prulière-Escabasse V, Wohlhuter-Haddad A, Epaud R, Fanen P, Tarze A
British journal of pharmacology 2016 Jun;173(11):1728-41

Deep interactome profiling of membrane proteins by co-interacting protein identification technology.

Pankow S, Bamberger C, Calzolari D, Bamberger A, Yates JR 3rd
Nature protocols 2016 Dec;11(12):2515-2528

Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.

Ferru-Clément R, Fresquet F, Norez C, Métayé T, Becq F, Kitzis A, Thoreau V
PloS one 2015;10(3):e0118943

CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction.

Tabeling C, Yu H, Wang L, Ranke H, Goldenberg NM, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke AC, Suttorp N, Proia RL, Witzenrath M, Kuebler WM
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 31;112(13):E1614-23

Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water.

Li Z, Lui EY, Wilson JM, Ip YK, Lin Q, Lam TJ, Lam SH
PloS one 2014;9(1):e87591

Correcting the cystic fibrosis disease mutant, A455E CFTR.

Cebotaru L, Rapino D, Cebotaru V, Guggino WB
PloS one 2014;9(1):e85183

Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.

Losa D, Köhler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong SS, Morel S, Nguyen TH, van Delden C, Chanson M
Journal of immunology (Baltimore, Md. : 1950) 2014 May 15;192(10):4804-12

Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels.

Hassan F, Xu X, Nuovo G, Killilea DW, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka PN, Cormet-Boyaka E
Respiratory research 2014 Jun 23;15:69

Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ΔF508-cystic fibrosis transmembrane conductance regulator (ΔF508-CFTR).

Caohuy H, Yang Q, Eudy Y, Ha TA, Xu AE, Glover M, Frizzell RA, Jozwik C, Pollard HB
The Journal of biological chemistry 2014 Dec 26;289(52):35953-68

Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells.

Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M
Journal of cellular and molecular medicine 2014 Aug;18(8):1631-43

Phosphorylated C/EBPβ influences a complex network involving YY1 and USF2 in lung epithelial cells.

Viart V, Varilh J, Lopez E, René C, Claustres M, Taulan-Cadars M
PloS one 2013;8(4):e60211

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).

El Khouri E, Le Pavec G, Toledano MB, Delaunay-Moisan A
The Journal of biological chemistry 2013 Oct 25;288(43):31177-91

Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in ΔF508 cystic fibrosis airway epithelium.

Oglesby IK, Chotirmall SH, McElvaney NG, Greene CM
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3354-62

Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.

Murphy SV, Lim R, Heraud P, Cholewa M, Le Gros M, de Jonge MD, Howard DL, Paterson D, McDonald C, Atala A, Jenkin G, Wallace EM
PloS one 2012;7(9):e46533

Proteomic identification of calumenin as a G551D-CFTR associated protein.

Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouvé P, Férec C
PloS one 2012;7(6):e40173

Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase.

King JD Jr, Lee J, Riemen CE, Neumann D, Xiong S, Foskett JK, Mehta A, Muimo R, Hallows KR
The Journal of biological chemistry 2012 Sep 28;287(40):33389-400

Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.

Duan Y, Sun Y, Zhang F, Zhang WK, Wang D, Wang Y, Cao X, Hu W, Xie C, Cuppoletti J, Magin TM, Wang H, Wu Z, Li N, Huang P
The Journal of biological chemistry 2012 Nov 23;287(48):40547-59

Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.

Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verzè G, Assael BM, Melotti P
PloS one 2011;6(7):e22212

Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.

Fouassier L, Rosenberg P, Mergey M, Saubaméa B, Clapéron A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
The American journal of pathology 2009 Mar;174(3):869-80

Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing.

Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB
The Journal of biological chemistry 2008 Aug 8;283(32):21926-33

Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries.

Robert R, Savineau JP, Norez C, Becq F, Guibert C
The European respiratory journal 2007 Nov;30(5):857-64

Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone.

Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE
Thorax 2007 Jul;62(7):650-1

Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones.

Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ
The Journal of endocrinology 2007 Jan;192(1):249-60

The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo.

Tang JY, Wong CK, Au DW
Biochemical and biophysical research communications 2007 Feb 2;353(1):98-103

Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.

Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
American journal of respiratory cell and molecular biology 2006 Jul;35(1):72-83

CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis.

Painter RG, Valentine VG, Lanson NA Jr, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G, Wang G
Biochemistry 2006 Aug 29;45(34):10260-9

Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells.

Nanua S, Sajjan U, Keshavjee S, Hershenson MB
Biochemical and biophysical research communications 2006 Apr 28;343(1):135-43

Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype.

Clain J, Lehmann-Che J, Duguépéroux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P
Human mutation 2005 Apr;25(4):360-71

Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.

Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
Journal of cell science 2004 Apr 15;117(Pt 10):1923-35

Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models.

Pan J, Bear C, Farragher S, Cutz E, Yeger H
American journal of respiratory cell and molecular biology 2002 Nov;27(5):553-60

Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.

Willumsen NJ, Amstrup J, Møbjerg N, Jespersen A, Kristensen P, Larsen EH
Biochimica et biophysica acta 2002 Nov 13;1566(1-2):28-43

Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.

Willumsen NJ, Amstrup J, Møbjerg N, Jespersen A, Kristensen P, Larsen EH
Biochimica et biophysica acta 2002 Nov 13;1566(1-2):28-43

Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.

Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
The Journal of biological chemistry 2002 Jun 14;277(24):21480-8

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.