MA1-935

antibody from Invitrogen Antibodies

Targeting: CFTR ABC35, ABCC7, CF, CFTR/MRP, dJ760C5.1, MRP7, TNR-CFTR

Western blot

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

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Validation data

Product number: MA1-935 - Provider product page
Provider: Invitrogen Antibodies
Product name: CFTR Monoclonal Antibody (CF3)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues. MA1-935 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a single ~170 kDa protein representing CFTR in T84 whole cell extract. Immunofluorescence staining of CFTR in mouse epithelial cells with MA1-935 results in cell surface staining, consistent with localization at the plasma membrane. This antibody also detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA. MA1-935 can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines. MA1-935 immunizing peptide corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgM
Antibody clone number: CF3
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

CFTR protein structure - MA1-935 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product # MA1-935) shows staining in HeLa cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product # MA1-935) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product # MA1-935) shows staining in U251 glioma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product # MA1-935) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product # MA1-935) shows staining in WiDr colon carcinoma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product # MA1-935) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.