16210-1-AP

antibody from Proteintech Group

Targeting: ASS1 ASS, CTLN1

Provider product page for 16210-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [43]
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Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 16210-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16210-1-AP, RRID:AB_2060466
Product name: ASS1 antibody
Antibody type: Polyclonal
Description: KD/KO validated ASS1 antibody (Cat. #16210-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ASS1 protein structure - 16210-1-AP shown in red.

Submitted references Microglial GLUL loss worsens TBI outcomes by amplifying the arginine-citrulline pathway.

Li J, Zhao Y, Yang Y, Dai Q, Tian R, Wang J, Ye J, Zhang L, Li L
Biochimica et biophysica acta. Molecular basis of disease 2026 Jun;1872(5):168230

Kaiyujiangzhuo formula alleviates glycolipid metabolic disorders by improving arginine metabolism and inhibiting the ERK-mediated PPARγ-Ser273 phosphorylation.

Shi Y, Zhang Z, Lin H, Wang S, Wang D, Wu H, Qi H, Wang Y, Liu Q, Zhang Y, Xiong H, Ren J, Li X, Wang Z
Journal of ethnopharmacology 2026 Jun 12;364:121508

Dysregulation of argininosuccinate synthase 1, phosphoenolpyruvate carboxykinase 1, and carbonyl reductase 3: Key arginine-proline metabolic mediators in mesangioproliferative glomerulopathies.

He J, Peng F, Qu Y, Zhao Y, Li Y, Zhou Y, Guo C, Li X, Song M, Chen X, Hong Q, Wang W
International journal of biological macromolecules 2026 Jan;337(Pt 2):149479

Functional studies and molecular dynamics reveal BPP-BrachyNH(2)-induced relaxation in male rat small mesenteric arteries via binding to argininosuccinate synthase.

Rufino Arcanjo DD, Meneses Bezerra IB, Comerma-Steffensen SG, Prat-Duran J, Moreira DC, Vasconcelos AG, Plácido A, Teixeira C, Gomes P, Cardoso-Teixeira AC, Coelho-de-Souza AN, Bemquerer M, Christensen BM, Wimmer R, Pereira de Oliveira A, Ramos RM, Simonsen U, de Souza de Almeida Leite JR
European journal of pharmacology 2025 Sep 5;1002:177857

Crataegus pinnatifida polysaccharide alleviates DSS-induced colitis in mice by regulating the intestinal microbiota and enhancing arginine biosynthesis.

Wei FH, Xie WY, Zhao PS, Ji ZH, Gao F, Chen CZ, Zhang Z, Gao W, Yuan B
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul;142:156794

Metabolomics and proteomics reveal blocking argininosuccinate synthetase 1 alleviates colitis in mice.

Liu S, Sun H, Du Z, Lu S, Wang C, Zhang Y, Luo Z, Wang L, Fan Z, Wei P, Yan Y, Zhang J, Yin S, Liu T, He Q, Guo X, Ding K, Zhou J, Hua H, Yu C, Xu W, Shan J, Li Y, Xu Y, Shen X, Cao G, Zhou W
Nature communications 2025 Jul 30;16(1):6983

Exogenous arginine differentially regulates inflammatory cytokine and inducible nitric oxide synthase expression in macrophages.

Stayer K, Pathan S, Biswas A, Li H, Zhu Y, Lam FW, Marini J, Thevananther S
ImmunoHorizons 2025 Jul 14;9(8)

The Mechanism of TRIM21 Inhibiting the Invasion and Migration of ccRCC by Stabilizing ASS1.

Yang Z, Cai J, Li J, Liu X, Liu W, Cui K, Bai Z, Dong Y, Peng D, Duan Q, Shahzad A, Zhang Q
Molecular carcinogenesis 2025 Feb;64(2):260-278

SENP3 promotes renal tubular epithelial cell apoptosis after ischemia-reperfusion injury via ASS1 deSUMOylation.

Wang H, Gao C, Yang J, Jin L, Wu L, Zhang Q, Fang X, Pan H, Wu H, Tangtanatakul P, Lin W, Yang Y
Cell death & disease 2025 Dec 5;17(1):76

Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants.

Yang C, Pataskar A, Feng X, Montenegro Navarro J, Paniagua I, Jacobs JJL, Zaal EA, Berkers CR, Bleijerveld OB, Agami R
Molecular cell 2024 May 16;84(10):1904-1916.e7

β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancer.

Wang H, Liu J, Zhang Z, Peng J, Wang Z, Yang L, Wang X, Hu S, Hong L
Free radical biology & medicine 2024 Mar;214:137-157

Quantitative Proteomics Provided Insights into the Protective Effects of Heat Acclimation on the Rat Hypothalamus after Exertional Heatstroke.

Xv F, Ma LZ, Li X, Zhao JB, Liu SY, Mao HD, Ma J, Xing L, Wang LF, Zhi WJ, Song Q
Journal of integrative neuroscience 2024 Jun 14;23(6):116

Endothelial cell Ass1 inhibits arteriosclerotic calcification in diabetes mellitus.

Jiang H, Zhou Y, Zhang L, Yu W, Li L, Dai Z, Zhao L, Wang Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Dec;181:117739

Cardiac Urea Cycle Activation by Time-Restricted Feeding Protects Against Pressure Overload-Induced Heart Failure.

Tan Y, Li M, Li H, Guo Y, Zhang B, Wu G, Li J, Zhang Q, Sun Y, Gao F, Yi W, Zhang X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Dec;11(48):e2407677

Single-cell RNA sequencing of pediatric renal tissues revealed the potential relationship between immunoglobulin A nephropathy and immunoglobulin A vasculitis with nephritis.

Ye Q, Meng H, Ye F, Fu H, Wang J, Liu F, Shen H, Bao Y, Feng C, Zhang X, Huang G, Xie Y, Zhu X, Zhao M, Guo G, Mao J
Clinical and translational medicine 2024 Apr;14(4):e1651

A pan-cancer analysis of the role of argininosuccinate synthase 1 in human tumors.

Ding Q, Li R, Wang Q, Yu L, Zi F
Frontiers in oncology 2023;13:1049147

The therapeutic effect of Shenhua tablet against mesangial cell proliferation and renal inflammation in mesangial proliferative glomerulonephritis.

He J, Peng F, Chang J, Zhao Y, Qu Y, Liu J, Liu R, Li P, Cai G, Hong Q, Chen X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Sep;165:115233

ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Hu Q, Dai J, Zhang Z, Yu H, Zhang J, Zhu X, Qin Y, Zhang L, Zhang P
Cancer research 2023 May 15;83(10):1646-1665

PSMD2 contributes to the progression of esophageal squamous cell carcinoma by repressing autophagy.

Liu Y, Wu M, Xu S, Niu X, Liu W, Miao C, Lin A, Xu Y, Yu L
Cell & bioscience 2023 Mar 30;13(1):67

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

Hepatocyte-derived MANF mitigates ethanol-induced liver steatosis in mice via enhancing ASS1 activity and activating AMPK pathway.

Xu HY, Jiao YH, Li SY, Zhu X, Wang S, Zhang YY, Wei YJ, Shen YJ, Wang W, Shen YX, Shao JT
Acta pharmacologica Sinica 2023 Jan;44(1):157-168

RNA-binding protein SPEN controls hepatocyte maturation via regulating Hnf4α expression during liver development.

Zhang J, Yang Z, Yan X, Duan J, Ruan B, Zhang X, Wen T, Zhang P, Liang L, Han H
Biochemical and biophysical research communications 2023 Jan 29;642:128-136

Hepatocyte-Specific Knock-Out of Nfib Aggravates Hepatocellular Tumorigenesis via Enhancing Urea Cycle.

Zhou L, Wang QL, Mao LH, Chen SY, Yang ZH, Liu X, Gao YH, Li XQ, Zhou ZH, He S
Frontiers in molecular biosciences 2022;9:875324

WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma.

Chu YD, Liu HF, Chen YC, Chou CH, Yeh CT
Frontiers in oncology 2022;12:996820

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin.

Head PE, Myung S, Chen Y, Schneller JL, Wang C, Duncan N, Hoffman P, Chang D, Gebremariam A, Gucek M, Manoli I, Venditti CP
Science translational medicine 2022 May 25;14(646):eabn4772

PGAM1 regulation of ASS1 contributes to the progression of breast cancer through the cAMP/AMPK/CEBPB pathway.

Liu M, Li R, Wang M, Liu T, Zhou Q, Zhang D, Wang J, Shen M, Ren X, Sun Q
Molecular oncology 2022 Aug;16(15):2843-2860

Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy.

Chu CY, Lee YC, Hsieh CH, Yeh CT, Chao TY, Chen PH, Lin IH, Hsieh TH, Shih JW, Cheng CH, Chang CC, Lin PS, Huang YL, Chen TM, Yen Y, Ann DK, Kung HJ
Theranostics 2021;11(8):3624-3641

BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.

Springer MZ, Poole LP, Drake LE, Bock-Hughes A, Boland ML, Smith AG, Hart J, Chourasia AH, Liu I, Bozek G, Macleod KF
Autophagy 2021 Nov;17(11):3530-3546

Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.

Marable SS, Chung E, Park JS
Journal of the American Society of Nephrology : JASN 2020 Nov;31(11):2543-2558

ASS1 Overexpression: A Hallmark of Sonic Hedgehog Hepatocellular Adenomas; Recommendations for Clinical Practice.

Sala M, Gonzales D, Leste-Lasserre T, Dugot-Senant N, Paradis V, Di Tommaso S, Dupuy JW, Pitard V, Dourthe C, Sciarra A, Sempoux C, Ferrell LD, Clouston AD, Miller G, Yeh MM, Thung S, Gouw ASH, Quaglia A, Han J, Huan J, Fan C, Crawford J, Nakanuma Y, Harada K, le Bail B, Castain C, Frulio N, Trillaud H, Possenti L, Blanc JF, Chiche L, Laurent C, Balabaud C, Bioulac-Sage P, Raymond AA, Saltel F
Hepatology communications 2020 Jun;4(6):809-824

An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.

Li WJ, Zhu XJ, Yuan TJ, Wang ZY, Bian ZQ, Jing HS, Shi X, Chen CY, Fu GB, Huang WJ, Shi YP, Liu Q, Zeng M, Zhang HD, Wu HP, Yu WF, Zhai B, Yan HX
Science translational medicine 2020 Jul 8;12(551)

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Lou F, Sun Y, Xu Z, Niu L, Wang Z, Deng S, Liu Z, Zhou H, Bai J, Yin Q, Cai X, Sun L, Wang H, Li Q, Wu Z, Chen X, Gu J, Shi YL, Tao W, Ginhoux F, Wang H
Immunity 2020 Jul 14;53(1):204-216.e10

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Zhang W, Dong Z, Xu M, Zhang S, Liu C, Chen S
Molecular metabolism 2020 Feb;32:85-96

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Cao Y, Ding W, Zhang J, Gao Q, Yang H, Cao W, Wang Z, Fang L, Du R
Journal of proteome research 2019 May 3;18(5):2032-2044

Argininosuccinate synthase 1 suppresses cancer cell invasion by inhibiting STAT3 pathway in hepatocellular carcinoma.

Tao X, Zuo Q, Ruan H, Wang H, Jin H, Cheng Z, Lv Y, Qin W, Wang C
Acta biochimica et biophysica Sinica 2019 Mar 1;51(3):263-276

Circular RNA circASS1 is downregulated in breast cancer cells MDA-MB-231 and suppressed invasion and migration.

Hou JC, Xu Z, Zhong SL, Zhang HD, Jiang LH, Chen X, Zhu LP, Li J, Zhou SY, Yang SJ, He YJ, Wang DD, Deng F, Zhang Q, Wang JY, Hu JH, Zhang W, Wu Y, Ding L, Zhao JH, Tang JH
Epigenomics 2019 Feb;11(2):199-213

Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.

Hong R, Zhang W, Xia X, Zhang K, Wang Y, Wu M, Fan J, Li J, Xia W, Xu F, Chen J, Wang S, Zhan Q
Molecular oncology 2019 Apr;13(4):959-977

Androgen receptor regulates ASS1P3/miR-34a-5p/ASS1 signaling to promote renal cell carcinoma cell growth.

Wang K, Sun Y, Guo C, Liu T, Fei X, Chang C
Cell death & disease 2019 Apr 18;10(5):339

Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.

Marable SS, Chung E, Adam M, Potter SS, Park JS
JCI insight 2018 Jul 26;3(14)

Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics for the investigation of the effect of Hugan Qingzhi on non-alcoholic fatty liver disease in rats.

Yao X, Xia F, Tang W, Xiao C, Yang M, Zhou B
Journal of ethnopharmacology 2018 Feb 15;212:208-215

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.

Lin R, Mo Y, Zha H, Qu Z, Xie P, Zhu ZJ, Xu Y, Xiong Y, Guan KL
Molecular cell 2017 Oct 5;68(1):198-209.e6

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Qin S, Zhou Y, Gray L, Kusebauch U, McEvoy L, Antoine DJ, Hampson L, Park KB, Campbell D, Caballero J, Glusman G, Yan X, Kim TK, Yuan Y, Wang K, Rowen L, Moritz RL, Omenn GS, Pirmohamed M, Hood L
Journal of proteome research 2016 Oct 7;15(10):3724-3740

Novel proteomic biomarker panel for prediction of aggressive metastatic hepatocellular carcinoma relapse in surgically resectable patients.

Tan GS, Lim KH, Tan HT, Khoo ML, Tan SH, Toh HC, Ching Ming Chung M
Journal of proteome research 2014 Nov 7;13(11):4833-46

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 16210-1-AP(ASS1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human testis using 16210-1-AP(ASS1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The ASS1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ASS1. This antibody recognizes human,mouse,rat antigen. The ASS1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 16210-1-AP.pdf

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