MA5-50643

antibody from Invitrogen Antibodies

Targeting: ASS1 ASS, CTLN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50643

Provider

Invitrogen Antibodies

Product name

ASS1 Recombinant Mouse Monoclonal Antibody (11F1-RA)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive control: HepG2 cell lysate, THP-1 cell lysate, SiHa cell lysate, rat kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue, human kidney tissue, MCF-7. Predicted band size: 47 kDa Subcellular Location: Cytoplasm.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

11F1-RA

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASS1 in different various lysates. Lane 1: HepG2 cell lysate (10 µg/Lane); Lane 2: THP-1 cell lysate (10 µg/Lane); Lane 3: SiHa cell lysate (10 µg/Lane); Lane 4: Rat kidney tissue lysate (20 µg/Lane). Predicted band size: 47 kDa. Observed band size: 47 kDa. Exposure time: 1 minute;12% SDS-PAGE gel. Primary antibody ASS1 recombinant monoclonal antibody (Product # MA5-50643) with a dilution of 1:5,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:150,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASS1 in different various lysates. Lane 1: Mouse liver tissue lysate (20 µg/Lane); Lane 2: Mouse kidney tissue lysate (20 µg/Lane). Predicted band size: 47 kDa. Observed band size: 47 kDa. Exposure time: 30 seconds; 12% SDS-PAGE gel. Primary antibody ASS1 recombinant monoclonal antibody (Product # MA5-50643) with a dilution of 1:20,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:150,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ASS1 in MCF-7 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with ASS1 recombinant monoclonal antibody (Product # MA5-50643) with a dilution of 1:200 in 2% negative goat serum overnight at 4 ℃. Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI, beta Tubulin was stained at a dilution of 1:100 overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of ASS1 in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with ASS1 recombinant monoclonal antibody (Product # MA5-50643) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of ASS1 in paraffin-embedded rat kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with ASS1 recombinant monoclonal antibody (Product # MA5-50643) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.