Antibody data
- Product number
- MA5-24297
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RCOR1 Monoclonal Antibody (658526)
- Provider product page
- Invitrogen Antibodies - MA5-24297
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human RCOR3 is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 658526
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of human RCOR1/CoREST Monoclonal Antibody (Product # MA5-24297) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for RCOR1/CoREST at approximately 64-66 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-RCOR1 Monoclonal Antibody (658526), (Product # MA5-24297) and a 60 kDa band corresponding to RCOR1 was observed in the cell lines tested. Modified whole cell extracts (1%SDS) (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), HEL 92.1.7 (Lane 3) and K-562 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of RCOR1/CoREST was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using human RCOR1/CoREST Monoclonal Antibody (Product # MA5-24297) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive data in Antibodypedia
Data presented on provider website
