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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-41564 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCOR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: MVEKGPEVSG KRRGRNNAAA SASAAAASAA ASAACASPAA TAASGAAASS Sequence homology: Human: 100%; Mouse: 100%; Zebrafish: 90%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Nuclear S-nitrosylation impacts tissue regeneration in zebrafish.
Matrone G, Jung SY, Choi JM, Jain A, Leung HE, Rajapakshe K, Coarfa C, Rodor J, Denvir MA, Baker AH, Cooke JP
Nature communications 2021 Nov 1;12(1):6282
Nature communications 2021 Nov 1;12(1):6282
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 3 Role of S-nitrosylation of Kdm1a in tailfin regeneration in adult zebrafish. A MS/MS fragmentation spectrum for the Cys334-containing peptide of Kdm1a. Peptide sequence is shown at the top left of the spectrum, with the annotation of the identified matched amino terminus-containing ions (b ions) in black and the carboxyl terminus-containing ions (y ions) in red. The spectrum confirms the identity of the peptides CPLYEAN and the labeled C as S-nitrosylated cysteine. B Line graph reporting the quantification of Kdm1a S-nitrosylation (normalized by total Kdm1a) and Kdm1a activity during tailfin regeneration. C Western blotting (WB) for S-nitrosylated Kdm1a in uninjured and at 3, 5, and 10 dpa. Samples were previously immunoprecipitated (IP) for Kdm1a. IP with IgG and input were used as controls. D - F WB for Kdm1a, CoRest and NuRD complexes components following IP with Kdm1a antibody in tailfin uninjured or injured at 5 dpa. Dpa days post-amputation. N = 3 biological replicates.
