MA5-42822

antibody from Invitrogen Antibodies

Targeting: RCOR1 COREST, KIAA0071, RCOR

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42822

Provider

Invitrogen Antibodies

Product name

RCOR1 Recombinant Rabbit Monoclonal Antibody (9B3R7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HT-29, HepG2, C2C12. The target is usually found in the following locations: Nucleus. Immunogen sequence: MPAMVEKGPE VSGKRRGRNN AAASASAAAA SAAASAACAS PAATAASGAA ASSASAAAAS AAAAPNNGQN KSLAAAAPNG NSSSNSWEEG SSGSSSDEEH

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9B3R7

Vial size

100 µL

Concentration

1.15 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RCOR1 in 300 μg extracts of THP-1 cells. Samples were precipitated with 3 μg RCOR1 Monoclonal antibody (Product # MA5-42822). Western blot was performed from the immunoprecipitate using RCOR1 Monoclonal antibody (Product # MA5-42822) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RCOR1 in paraffin-embedded human appendix. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RCOR1 in paraffin-embedded rat lung. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RCOR1 in paraffin-embedded mouse brain. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of RCOR1 in extracts of HepG2 cells. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.