Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-42822 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCOR1 Recombinant Rabbit Monoclonal Antibody (9B3R7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: HT-29, HepG2, C2C12. The target is usually found in the following locations: Nucleus. Immunogen sequence: MPAMVEKGPE VSGKRRGRNN AAASASAAAA SAAASAACAS PAATAASGAA ASSASAAAAS AAAAPNNGQN KSLAAAAPNG NSSSNSWEEG SSGSSSDEEH
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9B3R7
- Vial size
- 100 µL
- Concentration
- 1.15 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RCOR1 in 300 μg extracts of THP-1 cells. Samples were precipitated with 3 μg RCOR1 Monoclonal antibody (Product # MA5-42822). Western blot was performed from the immunoprecipitate using RCOR1 Monoclonal antibody (Product # MA5-42822) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RCOR1 in paraffin-embedded human appendix. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RCOR1 in paraffin-embedded rat lung. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RCOR1 in paraffin-embedded mouse brain. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of RCOR1 in extracts of HepG2 cells. Samples were incubated with RCOR1 Monoclonal antibody (Product # MA5-42822) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.