Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-21356 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAFB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HET/SAF B using 30 µg of A) A431 and B) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HET/SAF B polyclonal antibody (Product # PA5-21356) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Scaffold attachment factor B1 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Scaffold attachment factor B1 Polyclonal antibody (Product # PA5-21356) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAFB Polyclonal Antibody (Product # PA5-21356). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Scaffold attachment factor B1 antibody SAFB Polyclonal Antibody (Product # PA5-21356) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HET/SAF B in paraformaldehyde-fixed HeLa cells using a HET/SAF B polyclonal antibody (Product # PA5-21356) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Scaffold attachment factor B1 antibody (Product # PA5-21356) at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Scaffold attachment factor B1 antibody (Product # PA5-21356) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Scaffold attachment factor B1 antibody (Product # PA5-21356) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Scaffold attachment factor B1 antibody (Product # PA5-21356) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Scaffold attachment factor B1 antibody (Product # PA5-21356) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.