PA5-97277

antibody from Invitrogen Antibodies

Targeting: MRPS16 CGI-132

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97277

Provider

Invitrogen Antibodies

Product name

MRPS16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEKL LGLAGFFPLH PMMITNAERL RRKRAREVLL ASQKTDAEAT DTEATET; Positive Samples: HeLa, Jurkat, SW620, HT-1080, MCF7, Mouse heart, Mouse kidney, Mouse liver, Rat brain; Cellular Location: Mitochondrion

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1.12 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRPS16 in extracts of various cell lines. Samples were incubated with MRPS16 Polyclonal antibody (Product # PA5-97277) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence (Immunocytochemistry) analysis of MRPS16 in U2OS cells. Samples were incubated with MRPS16 Polyclonal antibody (Product # PA5-97277) using a dilution of 1:100. Blue: DAPI for nuclear staining.