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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF5428 - Provider product page
- Provider
- R&D Systems
- Product name
- Human C4.4A/LYPD3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human C4.4A/LYPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse C4.4A is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O95274- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells.
Görtz M, Galli U, Longerich T, Zöller M, Erb U, Schemmer P
Oncology reports 2017 Nov;38(5):2697-2704
Oncology reports 2017 Nov;38(5):2697-2704
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human C4.4A/LYPD3 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
