Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB03668 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- DOM3Z antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of DOM3Z (arrow) using rabbit polyclonal DOM3Z Antibody (N-term Y88) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the DOM3Z gene.
- Sample type
- lysatestransiently transfected with the DOM3Z gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of DOM3Z antibody (N-term Y88) in MCF-7 cell lysates (35ug/lane). DOM3Z (arrow) was detected using the purified Pab.
- Sample type
- MCF-7 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-231 cells using DOM3Z Antibody (N-term Y88)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- MDA-231 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol