PAB23385

antibody from Abnova Corporation

Targeting: CHCHD1 C10orf34, FLJ25854

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23385

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23385, RRID:AB_11122610

Product name

CHCHD1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CHCHD1.

Antigen sequence

MATPSLRGRLARFGNPRKPVLKPNKPLILANRVGE
RRREKGEATCITEMSVMMACWKQN


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CHCHD1 polyclonal antibody (Cat # PAB23385) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CHCHD1 polyclonal antibody (Cat # PAB23385) at 1-4 ug/mL dilution shows positivity in nucleoli, nucleus but not nucleoli and mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with CHCHD1 polyclonal antibody (Cat # PAB23385) shows strong cytoplasmic positivity in glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)