Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-21712 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-21712, RRID:AB_626713
- Product name
- Anti-ATP1A1
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Rapidly acquired resistance to EGFR tyrosine kinase inhibitors in NSCLC cell lines through de-repression of FGFR2 and FGFR3 expression.
Regulation of renal proximal tubule Na-K-ATPase by prostaglandins.
Enrichment of distinct microfilament-associated and GTP-binding-proteins in membrane/microvilli fractions from lymphoid cells.
Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition.
Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2.
Is Kir6.1 a subunit of mitoK(ATP)?
Ware KE, Marshall ME, Heasley LR, Marek L, Hinz TK, Hercule P, Helfrich BA, Doebele RC, Heasley LE
PloS one 2010 Nov 29;5(11):e14117
PloS one 2010 Nov 29;5(11):e14117
Regulation of renal proximal tubule Na-K-ATPase by prostaglandins.
Herman MB, Rajkhowa T, Cutuli F, Springate JE, Taub M
American journal of physiology. Renal physiology 2010 May;298(5):F1222-34
American journal of physiology. Renal physiology 2010 May;298(5):F1222-34
Enrichment of distinct microfilament-associated and GTP-binding-proteins in membrane/microvilli fractions from lymphoid cells.
Hao JJ, Wang G, Pisitkun T, Patino-Lopez G, Nagashima K, Knepper MA, Shen RF, Shaw S
Journal of proteome research 2008 Jul;7(7):2911-27
Journal of proteome research 2008 Jul;7(7):2911-27
Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition.
Wang Y, Torres-Gonzalez M, Tripathy S, Botolin D, Christian B, Jump DB
Journal of lipid research 2008 Jul;49(7):1538-52
Journal of lipid research 2008 Jul;49(7):1538-52
Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2.
Li C, Chen H, Hu L, Xing Y, Sasaki T, Villosis MF, Li J, Nishita M, Minami Y, Minoo P
BMC molecular biology 2008 Jan 23;9:11
BMC molecular biology 2008 Jan 23;9:11
Is Kir6.1 a subunit of mitoK(ATP)?
Foster DB, Rucker JJ, Marbán E
Biochemical and biophysical research communications 2008 Feb 15;366(3):649-56
Biochemical and biophysical research communications 2008 Feb 15;366(3):649-56
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 113
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
