MA3-928-A488

antibody from Invitrogen Antibodies

Targeting: ATP1A1

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-928-A488

Provider

Invitrogen Antibodies

Product name

ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA3928A488 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.

Conjugate

Green dye

Antibody clone number

M7-PB-E9

Concentration

1 mg/mL

References

Submitted references

Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.

Hansen GH, Niels-Christiansen LL, Danielsen EM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2008 Jul;56(7):677-85

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Na/K ATPase Alpha (green) in A431 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-928-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Green dye