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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA3-928-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 647
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3928A647 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only. The MA3928A647 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- M7-PB-E9
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.
Hansen GH, Niels-Christiansen LL, Danielsen EM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2008 Jul;56(7):677-85
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2008 Jul;56(7):677-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Na/K ATPase Alpha (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-928-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Red dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Red dye
