Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Other assay [2]
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Validation data
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- Product number
- MA3-924 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP1A1 Monoclonal Antibody (9-A5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-924 detects the alpha 1 sodium/potassium ATPase in human, mouse, canine, rat and avian tissues.
- Antibody clone number
- 9-A5
- Concentration
- Conc. Not Determined
Submitted references Characterization of CFTR High Expresser cells in the intestine.
Lubiprostone targets prostanoid signaling and promotes ion transporter trafficking, mucus exocytosis, and contractility.
Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.
The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.
Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids.
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus.
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.
Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.
Distribution of (Na+ + K+)ATPase and sodium channels in skeletal muscle and electroplax.
Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.
Localization of sodium/potassium adenosine triphosphatase in multiple cell types of the murine nervous system with antibodies raised against the enzyme from kidney.
Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.
Monoclonal antibodies to rat Na+,K+-ATPase block enzymatic activity.
Jakab RL, Collaco AM, Ameen NA
American journal of physiology. Gastrointestinal and liver physiology 2013 Sep 15;305(6):G453-65
American journal of physiology. Gastrointestinal and liver physiology 2013 Sep 15;305(6):G453-65
Lubiprostone targets prostanoid signaling and promotes ion transporter trafficking, mucus exocytosis, and contractility.
Jakab RL, Collaco AM, Ameen NA
Digestive diseases and sciences 2012 Nov;57(11):2826-45
Digestive diseases and sciences 2012 Nov;57(11):2826-45
Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.
Kajino K, Nakamura I, Bamba H, Sawai T, Ogasawara K
Immunology 2007 Jan;120(1):28-37
Immunology 2007 Jan;120(1):28-37
The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.
Boesze-Battaglia K, Song H, Sokolov M, Lillo C, Pankoski-Walker L, Gretzula C, Gallagher B, Rachel RA, Jenkins NA, Copeland NG, Morris F, Jacob J, Yeagle P, Williams DS, Damek-Poprawa M
Biochemistry 2007 Feb 6;46(5):1256-72
Biochemistry 2007 Feb 6;46(5):1256-72
Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids.
Kong JQ, Meng J, Biser PS, Fleming WW, Taylor DA
The Journal of pharmacology and experimental therapeutics 2001 Sep;298(3):909-16
The Journal of pharmacology and experimental therapeutics 2001 Sep;298(3):909-16
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Venkatesan S, Petrovic A, Locati M, Kim YO, Weissman D, Murphy PM
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus.
Khoo KM, Han MK, Park JB, Chae SW, Kim UH, Lee HC, Bay BH, Chang CF
The Journal of biological chemistry 2000 Aug 11;275(32):24807-17
The Journal of biological chemistry 2000 Aug 11;275(32):24807-17
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Wilkinson VL, Warrier RR, Truitt TP, Nunes P, Gately MK, Presky DH
Journal of immunological methods 1996 Jan 16;189(1):15-24
Journal of immunological methods 1996 Jan 16;189(1):15-24
Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.
Simon FR, Leffert HL, Ellisman M, Iwahashi M, Deerinck T, Fortune J, Morales D, Dahl R, Sutherland E
The American journal of physiology 1995 Jul;269(1 Pt 1):C69-84
The American journal of physiology 1995 Jul;269(1 Pt 1):C69-84
Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.
Ting-Beall HP, Beall HC, Hastings DF, Friedman ML, Ball WJ Jr
FEBS letters 1990 Jun 4;265(1-2):121-5
FEBS letters 1990 Jun 4;265(1-2):121-5
Distribution of (Na+ + K+)ATPase and sodium channels in skeletal muscle and electroplax.
Ariyasu RG, Deerinck TJ, Levinson SR, Ellisman MH
Journal of neurocytology 1987 Aug;16(4):511-22
Journal of neurocytology 1987 Aug;16(4):511-22
Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.
Hubert JJ, Schenk DB, Skelly H, Leffert HL
Biochemistry 1986 Jul 15;25(14):4156-63
Biochemistry 1986 Jul 15;25(14):4156-63
Localization of sodium/potassium adenosine triphosphatase in multiple cell types of the murine nervous system with antibodies raised against the enzyme from kidney.
Ariyasu RG, Nichol JA, Ellisman MH
The Journal of neuroscience : the official journal of the Society for Neuroscience 1985 Oct;5(10):2581-96
The Journal of neuroscience : the official journal of the Society for Neuroscience 1985 Oct;5(10):2581-96
Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.
Schenk DB, Hubert JJ, Leffert HL
The Journal of biological chemistry 1984 Dec 10;259(23):14941-51
The Journal of biological chemistry 1984 Dec 10;259(23):14941-51
Monoclonal antibodies to rat Na+,K+-ATPase block enzymatic activity.
Schenk DB, Leffert HL
Proceedings of the National Academy of Sciences of the United States of America 1983 Sep;80(17):5281-5
Proceedings of the National Academy of Sciences of the United States of America 1983 Sep;80(17):5281-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on human brain tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on human renal carcinoma tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on human liver tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL