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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- ANP-001-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alpha 1 Na+/K+ ATPase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody , preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate: - 1. Anti-Alpha 1 Na+/K+ATPase Antibody (#ANP-001), (1:200). 2. Anti-Alpha 1 Na+/K+ATPase Antibody , preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).Note:Samples should be heated up to 37°C. Do not boil samples.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody , preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate: - 1. Anti-Alpha 1 Na+/K+ATPase Antibody (#ANP-001), (1:200). 2. Anti-Alpha 1 Na+/K+ATPase Antibody , preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).Note:Samples should be heated up to 37°C. Do not boil samples.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Na+/K+ATPase alpha 1 (ATP1A1) in mouse brain - Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Alpha 1 Na+/K+ATPase Antibody (#ANP-001), (1:400). ATP1A1staining (red) is apparent in the hippocampal dentate gyrus in neuronal soma (vertical arrows) and in astrocytes (horizontal arrow). DAPI (blue) is used to visualize cell nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Na+/K+ATPase alpha 1 (ATP1A1) in rat kidney - Immunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ATPase Antibody (#ANP-001), (1:200). ATP1A1staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.
