Ab088306

antibody from Aladdin Scientific

Targeting: ATP1A1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab088306

Provider

Aladdin Scientific

Product name

ATP1A1 Mouse mAb

Antibody type

Monoclonal

Description

Mouse monoclonal (C209) to ATP1A1 could be uesd for WB, IHC and so on.Application:Western blotting: 0.5-2µg/mLImmunohistochemistry: 5-20µg/mLProtein FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.Post-translationalPhosphorylation on Tyr-10 modulates pumping activity.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine, Rabbit

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

His493~Ala660


Isotype

IgG

Antibody clone number

C209

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C for frequent use. Stored at -20¡C in a manual defrost freezer for two year without detectable loss of activity. Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 117.2 s

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 30.0 s

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Porcine Kidney Tissue Primary Ab: 20 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Uterus Tissue Primary Ab: 3 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Small intestine Tissue Primary Ab: 3 ug/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.