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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- Ab128631 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant ATP1A1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human ATP1A1 Antibody, Recombinant antibody (RR685), could be used for WB, IHC, Flow, IP and so on.Application:WB: 1/2000 - 1/5000 ÊÊÊÊÊÊIHC: 1/10000 - 1/20000Flow: 1/50 - 1/200IP: 1/50Protein FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.Post-translationalPhosphorylation on Tyr-10 modulates pumping activity.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RR685
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ATP1A1 Antibody (Ab128631) - Western Blot All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 97 kDa Exposure time: 32.2 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ATP1A1 Antibody (Ab128631) - Western Blot All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 97 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ATP1A1 Antibody (Ab128631) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
