- MA1-16731 - Invitrogen Antibodies ATP1A1 antibody

Submitted references Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty.

Ali M, Khan SY, Gottsch JD, Hutchinson EK, Khan A, Riazuddin SA
Stem cell reports 2021 Sep 14;16(9):2320-2335

Changes in cellular Ca(2+) and Na(+) regulation during the progression towards heart failure in the guinea pig.

Ke HY, Yang HY, Francis AJ, Collins TP, Surendran H, Alvarez-Laviada A, Firth JM, MacLeod KT
The Journal of physiology 2020 Apr;598(7):1339-1359

A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.

Booth AE, Tarafder AK, Hume AN, Recchi C, Seabra MC
PloS one 2014;9(7):e102851

Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.

Wen X, Lacruz RS, Smith CE, Paine ML
European journal of oral sciences 2014 Feb;122(1):21-6

Altered Na+ transport after an intracellular alpha-subunit deletion reveals strict external sequential release of Na+ from the Na/K pump.

Yaragatupalli S, Olivera JF, Gatto C, Artigas P
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 8;106(36):15507-12

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of ATP1A1 in porcine proximal tubule protein. Sample was incubated in ATP1A1 monoclonal antibody (Product # MA1-16731).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry analysis of ATP1A1 in HepG2 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731). ATPA1 (Green). Nuclei (Blue) were counterstained using Hoechst 33258.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of ATP1A1 in HepG2 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731). ATPA1 (Green). Nuclei (Blue) were counterstained using Hoechst 33258.

Supportive validation

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Experimental details: Immunohistochemical analysis of ATP1A1 in human enodmetrial glands within the uterus. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731). Note the absence of staining in the surrounding myometrial smooth muscle.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection of Sodium Potassium ATPase Alpha 1 in fixed Hela cells using Product # MA1-16731.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry of ATP1A1 in A549 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731) and a matched isotype control using a dilution of 1 µg/mL for 30 minutes at room temperature followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Image using the Purified form of this antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of ATP1A1 in A549 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 Immunostaining of Na + , K + -ATPase (NKA) isoforms alpha1 ( Aa and Ba ) and alpha2 ( Ab and Bb ) Aa , cardiomyocyte staining of Na + ,K + -ATPase alpha1 in Sham and 150-day post-AC cells. Visibly reduced staining in AC cell compared to Sham. One ROI (shown by yellow rectangle 24 x 15 mum) was placed in the centre and another near the edge of cell to measure the total area of fluorescence and the mean total area of the two ROI was calculated. b , Na + ,K + -ATPase alpha2 immunolabelling in Sham and AC cells. Ba , total area of fluorescence of Na + ,K + -ATPase alpha1 markedly declines between Sham and AC. b , the total area of fluorescence of Na + ,K + -ATPase alpha2 is significantly reduced between age-matched Sham and AC groups. *** P < 0.001. Sham n = 22 and 150AC n = 46 for NKAalpha1, Sham n = 49 and 150AC n = 30 for NKAalpha2 from two hearts per group. Mean (SD).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 9 Western blot analyses of Na + , K + ATPase alpha1 and phospholemman between animal groups A , western blot of Na + ,K + -ATPase (NKA) alpha1 expression in Sham and AC groups at 60 and 150 days post-operatively. The Na + ,K + -ATPase alpha1 relative expression was significantly reduced in 60 and 150 days compared to their age-matched Sham groups. N = 5 for 60Sham and N = 4 for 60AC; N = 6 for 150Sham, N = 7 for 150AC. Mean (SD). Student''s t test one-tailed, * P < 0.05, ** P < 0.01. B , western blot of expression of phospholemman (PLM), a protein which regulates the activity of Na + ,K + -ATPase. The relative expression of PLM significantly decreases between 60-day sham and 60-day AC, but was unchanged between the 150-day AC group compared to age-matched Sham. Mean (SD). Student''s t test one-tailed, * P < 0.05. N = 5 for 60Sham and N = 7 for 60AC; N = 4 for 150Sham, N = 4 for 150AC.

MA1-16731

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