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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AMM85963 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- RAC1 Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity Purification
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right), using RAC1 Antibody. RAC1 Mouse Monoclonal Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H.skin section using RAC1(Cat#AMM85963). AMM85963 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H.stomach section using RAC1(Cat#AMM85963). AMM85963 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Flow cytometric analysis of U-87 MG cells using RAC1(green, Cat#AMM85963) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
