66122-1-Ig

antibody from Proteintech Group

Targeting: RAC1 p21-Rac1, Rac-1, TC-25

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

66122-1-Ig

Provider

Proteintech Group

Product name

Rac1 antibody

Antibody type

Monoclonal

Description

KD/KO validated RAC1 antibody (Cat. #66122-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

4A4B11

Vial size

20ul, 150ul

References

Submitted references

Midkine Overexpression Promotes Functional Recovery After Spinal Cord Injury by Enhancing Microglial Efferocytosis Via LRP-1.

Wang Y, Fang L, Zhai C, Cai J, Li X, Zong L, Geng Y, Zhu C, Sun C, Dong M, Qian Y, Liu Y, Huang Y, Wang Z, Wang T, Liu WT, Wu Q
CNS neuroscience & therapeutics 2026 Mar;32(3):e70841

---

Smoking-Induced STC2(+) Tumor Cells Drive Tumor-Vascular Crosstalk in Laryngeal Squamous Cell Carcinoma via Spatial and Single-Cell Transcriptomics.

Shen Y, Gao L, Huang Q, Zhou L, Xu C, Wu C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(5):e11932

---

RAC1 directly phosphorylates both PKM2 and FBP1 to promote radioresistance in hepatocellular carcinoma.

Jiang Y, Zhou K, Wei X, Feng J, Zhou B, Liu C, Sun J, Zhu H, Liu Z, Yuan N, Li Y, Meng X, Cao J, Jiao Y, Cheng S
Molecular therapy : the journal of the American Society of Gene Therapy 2026 Feb 4;34(2):1201-1214

---

Comprehensive Pan-Cancer Analysis of RAC1 Decoding the Impact on Cancer Prognosis and B cell immune Regulation.

Shao Z, Ren H, Wang X, Zhang Q, Xue J, Chi Y, Xiu B, Wu J
International journal of medical sciences 2025;22(3):662-679

---

LZTR1 is a melanoma oncogene that promotes invasion and suppresses apoptosis.

Bacchiocchi A, Mak M, Khan ZM, Gong X, Sznol M, Na Z, Su H, Chan LH, Yan Q, Zhao D, Mortlock RD, Knight J, Slavoff SA, Halaban R
Oncogene 2025 Oct;44(41):3974-3984

---

Interactive effects of Bisphenol F exposure and TLR4 rs4986790 on unexplained miscarriage.

Wang M, Huang W, Chen W, Yue X, Sun Y, Lin Y, Xing S, Zhao D, Wang Y, Guo G, Fan Q, Zhang Z, Cheng W, Huang X, Wu L, Zhang H
EBioMedicine 2025 Nov;121:105968

---

Lipid droplets restrict phagosome formation in antifungal immunity.

Sun W, Wu H, Zhao G, Shui Q, Zhang L, Luan X, Chen T, Liu F, Zheng Y, Zhao W, Qi X, Liu B, Gao C
Cellular & molecular immunology 2025 May;22(5):468-484

---

Treponema pallidum Protein TpF1 Inhibits Migration by Impairing Actin Polymerization via Toll-Like Receptor 4/PI3K/AKT in Microglia.

Zhao YY, Xie L, Wang RY, Yan Y, Liu LL
ACS infectious diseases 2025 May 9;11(5):1104-1113

---

RAC1 Regulates Shh-Medulloblastoma Growth via GLI-Mediated Transcription.

Jangde N, Lee MH, Ruiz L, Egan I, Jermakowicz AM, Wynn D, Goka E, Lippman M, Robbins DJ, Ayad NG
bioRxiv : the preprint server for biology 2025 May 23;

---

CXCL12/CXCR4 modulates macrophage efferocytosis to induce glomerular crescent formation and fibrosis via ELMO1/DOCK180/RAC1 signaling in ANCA-associated glomerulonephritis.

Liu Z, Deng Y, Song X, Cai X, Xiong H, Tan L, Wei S, Li Q, Wang X, Jiang W, Chen Y, Li Q, Wang M
Cellular and molecular life sciences : CMLS 2025 Jul 19;82(1):280

---

Single-Cell Analysis of Endothelial Cell Injury in IgA Nephropathy.

Yang YC, Zhu L, Zhao JY, Zhang B, Wang XM, Cheung WW, Zhao CG, Zhou P
Immunity, inflammation and disease 2025 Feb;13(2):e70149

---

Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation.

Qiu J, Qu Y, Li Y, Li C, Wang J, Meng L, Jing X, Fu J, Xu Y, Chai Y
Biology direct 2025 Feb 8;20(1):21

---

Human neuron chimeric mice reveal impairment of DVL-1-mediated neuronal migration by sevoflurane and potential treatment by rTMS.

Zhao Y, Zhao Y, Liang L, Chen A, Li Y, Liu K, Xie R, Mao H, Ren B, Huang B, Shi C, Shao Z, Wu S, Wang Y, Zhang H
Experimental & molecular medicine 2025 Apr;57(4):745-758

---

Liraglutide modulates ALCAT1-Mediated cardiolipin remodeling to improve cardiac function in obese mice.

Wang L, Hu T, Zhang R, Shi Y, Wang Y, Xuan Q, Zhou X
Biochemical and biophysical research communications 2025 Apr 5;756:151583

---

Pathogenesis of Focal Segmental Glomerulosclerosis Caused by a Leu754Val Mutation in ARHGAP32.

Chen S, Wang L, Zou Y, Li Y, Zhong X, Li G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Apr 30;39(8):e70534

---

RBM5 induces motor neuron apoptosis in hSOD1(G93A)-related amyotrophic lateral sclerosis by inhibiting Rac1/AKT pathways.

Tan X, Su X, Wang Y, Liang W, Wang D, Huo D, Wang H, Qi Y, Zhang W, Han L, Zhang D, Wang M, Xu J, Feng H
Brain research bulletin 2024 Oct 1;216:111049

---

Hypoxia Stimulates PYGB Enzymatic Activity to Promote Glycogen Metabolism and Cholangiocarcinoma Progression.

Pan Y, Zhou Y, Shen Y, Xu L, Liu H, Zhang N, Huang T, Meng K, Liu Y, Wang L, Bai G, Chen Q, Zhu Y, Zou X, Wang S, Wang Z, Wang L
Cancer research 2024 Nov 15;84(22):3803-3817

---

Spatiotemporal dynamics exhibited by horizontal basal cells reveal a pro-neurogenic pathway during injury-induced olfactory epithelium regeneration.

Louie JD, Barrios-Camacho CM, Bromberg BH, Hintschich CA, Schwob JE
iScience 2024 May 17;27(5):109600

---

Tiam1 regulates proliferation, invasion, and differentiation in neuroblastoma through the Tiam1/Rac1 signaling pathway.

Li X, Liu B, Wang X, Zhuang Y, Gao T, Sun N
Neoplasma 2024 Feb;71(1):37-47

---

RAC1 inhibition ameliorates IBSP-induced bone metastasis in lung adenocarcinoma.

Zhang X, Liang X, Wen Y, Wu F, Gao G, Zhang L, Gu Y, Zhang J, Zhou F, Li W, Tang L, Yang X, Zhao H, Zhou C, Hirsch FR
Cell reports 2024 Aug 27;43(8):114528

---

Autism Spectrum Disorder- and/or Intellectual Disability-Associated Semaphorin-5A Exploits the Mechanism by Which Dock5 Signalosome Molecules Control Cell Shape.

Okabe M, Sato T, Takahashi M, Honjo A, Okawa M, Ishida M, Kukimoto-Niino M, Shirouzu M, Miyamoto Y, Yamauchi J
Current issues in molecular biology 2024 Apr 2;46(4):3092-3107

---

Caffeic acid phenethyl ester ameliorates titanium particle-induced bone loss and inflammatory reaction in a mouse acute model.

Xu X, Li L, Wang B, Shi B
Biochemical and biophysical research communications 2023 Nov 12;681:47-54

---

TRPM7 Kinase Domain is Part of the Rac1-SSH2-cofilin Complex Regulating F-actin in the Mouse Nervous System.

Chang J, Chen C, Li W, Abumaria N
Neuroscience bulletin 2023 Jun;39(6):989-993

---

Long Noncoding RNA AC078850.1 Induces NLRP3 Inflammasome-Mediated Pyroptosis in Atherosclerosis by Upregulating ITGB2 Transcription via Transcription Factor HIF-1α.

Tian Y, Luo Q, Huang K, Sun T, Luo S
Biomedicines 2023 Jun 16;11(6)

---

DIRAS3 enhances RNF19B-mediated RAC1 ubiquitination and degradation in non-small-cell lung cancer cells.

Wang Y, Wei M, Su M, Du Z, Dong J, Zhang Y, Wu Y, Li X, Su L, Liu X
iScience 2023 Jul 21;26(7):107157

---

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis.

Zhou W, Liu H, Yuan Z, Zundell J, Towers M, Lin J, Lombardi S, Nie H, Murphy B, Yang T, Wang C, Liao L, Goldman AR, Kannan T, Kossenkov AV, Drapkin R, Montaner LJ, Claiborne DT, Zhang N, Wu S, Zhang R
Cancer cell 2023 Apr 10;41(4):740-756.e10

---

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.

Liu D, Lin S, Li Y, Zhou T, Hu K, Li Q
Drug design, development and therapy 2022;16:375-395

---

Single-cell RNA sequencing of CSF reveals neuroprotective RAC1(+) NK cells in Parkinson's disease.

Guan Q, Liu W, Mu K, Hu Q, Xie J, Cheng L, Wang X
Frontiers in immunology 2022;13:992505

---

New mechanism for mesenchymal stem cell microvesicle to restore lung permeability: intracellular S1P signaling pathway independent of S1P receptor-1.

Ye L, Song J, Zheng Y, Zhong M, Liu J, Zhu D, Hu S
Stem cell research & therapy 2022 Oct 8;13(1):496

---

The gut microbe Bacteroides fragilis ameliorates renal fibrosis in mice.

Zhou W, Wu WH, Si ZL, Liu HL, Wang H, Jiang H, Liu YF, Alolga RN, Chen C, Liu SJ, Bian XY, Shan JJ, Li J, Tan NH, Zhang ZH
Nature communications 2022 Oct 14;13(1):6081

---

SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion.

Zhang YY, Liang R, Wang SJ, Ye ZW, Wang TY, Chen M, Liu J, Na L, Yang YL, Yang YB, Yuan S, Yin X, Cai XH, Tang YD
The Journal of biological chemistry 2022 Nov;298(11):102511

---

SYTL5 Promotes Papillary Thyroid Carcinoma Progression by Enhancing Activation of the NF-κB Signaling Pathway.

Huang D, Zeng Y, Deng HY, Fu BD, Ke Y, Luo JY, Yu JC, Yu ST
Endocrinology 2022 Nov 14;164(1)

---

Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

Ni L, Lin B, Hu L, Zhang R, Fu F, Shen M, Yang J, Shi D
Journal of the American Heart Association 2022 Jun 7;11(11):e024854

---

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Wolff DW, Deng Z, Bianchi-Smiraglia A, Foley CE, Han Z, Wang X, Shen S, Rosenberg MM, Moparthy S, Yun DH, Chen J, Baker BK, Roll MV, Magiera AJ, Li J, Hurley E, Feltri ML, Cox AO, Lee J, Furdui CM, Liu L, Bshara W, LaConte LEW, Kandel ES, Pasquale EB, Qu J, Hedstrom L, Nikiforov MA
Cell chemical biology 2022 Jun 16;29(6):970-984.e6

---

Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.

Han K, Wang F, Yue Y, Tan X, Tian M, Miao Y, Zhao S, Dong W, Yu M
Cell proliferation 2022 Jan;55(1):e13169

---

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Jin X, Zhang B, Zhang H, Yu H
Oncogene 2022 Jan;41(4):538-549

---

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin.

Alalem M, Bhosale M, Ranjan A, Yamamoto S, Kaida A, Nishikawa S, Parrales A, Farooki S, Anant S, Padhye S, Iwakuma T
Cancers 2022 Aug 29;14(17)

---

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8(+) memory T cells.

Zhang H, Liu J, Yang Z, Zeng L, Wei K, Zhu L, Tang L, Wang D, Zhou Y, Lv J, Zhou N, Tang K, Ma J, Huang B
Molecular cell 2022 Aug 18;82(16):3077-3088.e6

---

Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.

Ying C, Dai J, Fan G, Zhou Z, Gan T, Zhang Y, Song Y, Zhou X
Frontiers in physiology 2021;12:679166

---

Regulation of local GTP availability controls RAC1 activity and cell invasion.

Bianchi-Smiraglia A, Wolff DW, Marston DJ, Deng Z, Han Z, Moparthy S, Wombacher RM, Mussell AL, Shen S, Chen J, Yun DH, O'Brien Cox A, Furdui CM, Hurley E, Feltri ML, Qu J, Hollis T, Kengne JBN, Fongang B, Sousa RJ, Kandel ME, Kandel ES, Hahn KM, Nikiforov MA
Nature communications 2021 Oct 19;12(1):6091

---

Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.

Lyu L, Chen J, Wang W, Yan T, Lin J, Gao H, Li H, Lv R, Xu F, Fang L, Chen Y
Journal of cellular and molecular medicine 2021 Mar;25(6):3136-3148

---

TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.

Geng J, Shi Y, Zhang J, Yang B, Wang P, Yuan W, Zhao H, Li J, Qin F, Hong L, Xie C, Deng X, Sun Y, Wu C, Chen L, Zhou D
Nature communications 2021 Jun 10;12(1):3519

---

The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.

Hu X, Xiang L, He D, Zhu R, Fang J, Wang Z, Cao K
Clinical science (London, England : 1979) 2021 Feb 12;135(3):555-574

---

MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling.

Tang H, Wang X, Zhang M, Yan Y, Huang S, Ji J, Xu J, Zhang Y, Cai Y, Yang B, Lan W, Huang M, Zhang L
Cell death & disease 2020 Oct 29;11(10):931

---

Asiatic acid prevents renal fibrosis in UUO rats via promoting the production of 15d-PGJ2, an endogenous ligand of PPAR-γ.

Zhang ZH, He JQ, Zhao YY, Chen HC, Tan NH
Acta pharmacologica Sinica 2020 Mar;41(3):373-382

---

Kidney targeted delivery of asiatic acid using a FITC labeled renal tubular-targeting peptide modified PLGA-PEG system.

He J, Chen H, Zhou W, Chen M, Yao Y, Zhang Z, Tan N
International journal of pharmaceutics 2020 Jun 30;584:119455

---

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.

Huang D, Siaw-Debrah F, Wang H, Ye S, Wang K, Wu K, Zhang Y, Wang H, Yao C, Chen J, Yan L, Zhang CL, Zhuge Q, Yang J
Aging 2020 Dec 19;13(2):2822-2850

---

4-Octyl itaconate protects against renal fibrosis via inhibiting TGF-β/Smad pathway, autophagy and reducing generation of reactive oxygen species.

Tian F, Wang Z, He J, Zhang Z, Tan N
European journal of pharmacology 2020 Apr 15;873:172989

---

DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53.

Wudu M, Ren H, Hui L, Jiang J, Zhang S, Xu Y, Wang Q, Su H, Jiang X, Dao R, Qiu X
Journal of experimental & clinical cancer research : CR 2019 Feb 12;38(1):72

---

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Ma D, Zheng B, Suzuki T, Zhang R, Jiang C, Bai D, Yin W, Yang Z, Zhang X, Hou L, Zhan H, Wen JK
Circulation research 2017 Mar 3;120(5):799-815

---

The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocarcinoma cells.

Li J, Zhang S, Hu Q, Zhang K, Jin J, Zheng X, Yin Z, Wang X
Scientific reports 2016 May 27;6:26971

---

Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.

Zhou T, Zhang B, Wei P, Du Y, Zhou H, Yu M, Yan L, Zhang W, Nie G, Chen C, Tu Y, Wei T
Biomaterials 2014 Dec;35(37):9833-9843

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The Rac1 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human Rac1. This antibody recognizes human,mouse antigen. The Rac1 antibody has been validated for the following applications: ELISA, WB analysis.

Appendix

66122-1-Ig.pdf

Show more