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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [41]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- 11329-2-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11329-2-AP, RRID:AB_2238179
- Product name
- RAB35 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RAB35 antibody (Cat. #11329-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.
Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation.
DDX3 suppresses hepatocellular carcinoma progression through modulating the secretion and composition of exosome.
MiniBAR/GARRE1 is a dual Rac and Rab effector required for ciliogenesis.
miR-31-mediated local translation at the mitotic spindle is important for early development.
Chronic pain causes Tau-mediated hippocampal pathology and memory deficits.
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.
TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.
Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.
Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.
Improved Small Extracellular Vesicle Secretion of Rat Adipose-Derived Stem Cells by Microgrooved Substrates through Upregulation of the ESCRT-III-Associated Protein Alix.
LRRK2 mediates tubulation and vesicle sorting from lysosomes.
Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.
Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration.
NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.
LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation.
A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.
Rab35 GTPase recruits NDP52 to autophagy targets.
Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.
Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.
Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.
Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.
Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.
Identification of an oncogenic RAB protein.
Novel functions for the endocytic regulatory proteins MICAL-L1 and EHD1 in mitosis.
Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.
CLIC4 regulates cell adhesion and β1 integrin trafficking.
Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.
Syntaxin 16 is a master recruitment factor for cytokinesis.
TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes.
MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a.
Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35.
EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.
Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis.
Androgen-related expression of G-proteins in ovarian cancer.
Zhang KH, Zou YJ, Shan MM, Pan ZN, Ju JQ, Liu JC, Ji YM, Sun SC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(4):e2303009
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(4):e2303009
Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation.
Mellouk N, Lensen A, Lopez-Montero N, Gil M, Valenzuela C, Klinkert K, Moneron G, Swistak L, DiGregorio D, Echard A, Enninga J
Cell reports 2024 Apr 23;43(4):114034
Cell reports 2024 Apr 23;43(4):114034
DDX3 suppresses hepatocellular carcinoma progression through modulating the secretion and composition of exosome.
Su YY, Chao CH, Hsu HY, Li HK, Wang YL, Wu Lee YH, Mai RT
American journal of cancer research 2023;13(5):1744-1765
American journal of cancer research 2023;13(5):1744-1765
MiniBAR/GARRE1 is a dual Rac and Rab effector required for ciliogenesis.
Serres MP, Shaughnessy R, Escot S, Hammich H, Cuvelier F, Salles A, Rocancourt M, Verdon Q, Gaffuri AL, Sourigues Y, Malherbe G, Velikovsky L, Chardon F, Sassoon N, Tinevez JY, Callebaut I, Formstecher E, Houdusse A, David NB, Pylypenko O, Echard A
Developmental cell 2023 Nov 20;58(22):2477-2494.e8
Developmental cell 2023 Nov 20;58(22):2477-2494.e8
miR-31-mediated local translation at the mitotic spindle is important for early development.
Remsburg CM, Konrad K, Stepicheva N, Testa M, Lee K, Choe L, Polson S, Bhavsar J, Huang H, Song JL
Research square 2023 Jun 14;
Research square 2023 Jun 14;
Chronic pain causes Tau-mediated hippocampal pathology and memory deficits.
Guerreiro SR, Guimarães MR, Silva JM, Dioli C, Vamvaka-Iakovou A, Sousa R, Gomes P, Megalokonomou A, Campos-Marques C, Cunha AM, Almeida A, Sousa N, Leite-Almeida H, Sotiropoulos I
Molecular psychiatry 2022 Nov;27(11):4385-4393
Molecular psychiatry 2022 Nov;27(11):4385-4393
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.
Matsui T, Sakamaki Y, Nakashima S, Fukuda M
Cell reports 2022 May 31;39(9):110875
Cell reports 2022 May 31;39(9):110875
TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.
Zhang T, Zhao L, Han L, Li Y, Hu L, Wang H, Zou F
International journal of molecular sciences 2022 Jun 12;23(12)
International journal of molecular sciences 2022 Jun 12;23(12)
Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.
Iannantuono NVG, Emery G
Journal of cell science 2021 Jun 15;134(12)
Journal of cell science 2021 Jun 15;134(12)
Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.
Zhuravleva V, Vaz-Silva J, Zhu M, Gomes P, Silva JM, Sousa N, Sotiropoulos I, Waites CL
Cell death & disease 2021 Dec 8;12(12):1137
Cell death & disease 2021 Dec 8;12(12):1137
Improved Small Extracellular Vesicle Secretion of Rat Adipose-Derived Stem Cells by Microgrooved Substrates through Upregulation of the ESCRT-III-Associated Protein Alix.
Ji Y, Han W, Fu X, Li J, Wu Q, Wang Y
Advanced healthcare materials 2021 Aug;10(16):e2100492
Advanced healthcare materials 2021 Aug;10(16):e2100492
LRRK2 mediates tubulation and vesicle sorting from lysosomes.
Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, Cookson MR
Science advances 2020 Nov;6(46)
Science advances 2020 Nov;6(46)
Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.
Kinoshita R, Homma Y, Fukuda M
The Journal of biological chemistry 2020 Mar 13;295(11):3652-3663
The Journal of biological chemistry 2020 Mar 13;295(11):3652-3663
Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
Mukherjee C, Kling T, Russo B, Miebach K, Kess E, Schifferer M, Pedro LD, Weikert U, Fard MK, Kannaiyan N, Rossner M, Aicher ML, Goebbels S, Nave KA, Krämer-Albers EM, Schneider A, Simons M
Cell metabolism 2020 Aug 4;32(2):259-272.e10
Cell metabolism 2020 Aug 4;32(2):259-272.e10
Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.
Wallroth A, Koch PA, Marat AL, Krause E, Haucke V
Nature cell biology 2019 Sep;21(9):1093-1101
Nature cell biology 2019 Sep;21(9):1093-1101
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Clancy JW, Zhang Y, Sheehan C, D'Souza-Schorey C
Nature cell biology 2019 Jul;21(7):856-866
Nature cell biology 2019 Jul;21(7):856-866
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications 2019 Apr 8;10(1):1608
Nature communications 2019 Apr 8;10(1):1608
Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration.
Jeong GR, Jang EH, Bae JR, Jun S, Kang HC, Park CH, Shin JH, Yamamoto Y, Tanaka-Yamamoto K, Dawson VL, Dawson TM, Hur EM, Lee BD
Molecular neurodegeneration 2018 Feb 13;13(1):8
Molecular neurodegeneration 2018 Feb 13;13(1):8
NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.
Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N
EMBO reports 2018 Dec;19(12)
EMBO reports 2018 Dec;19(12)
LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation.
Bae EJ, Kim DK, Kim C, Mante M, Adame A, Rockenstein E, Ulusoy A, Klinkenberg M, Jeong GR, Bae JR, Lee C, Lee HJ, Lee BD, Di Monte DA, Masliah E, Lee SJ
Nature communications 2018 Aug 27;9(1):3465
Nature communications 2018 Aug 27;9(1):3465
A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.
Corallino S, Malinverno C, Neumann B, Tischer C, Palamidessi A, Frittoli E, Panagiotakopoulou M, Disanza A, Malet-Engra G, Nastaly P, Galli C, Luise C, Bertalot G, Pece S, Di Fiore PP, Gauthier N, Ferrari A, Maiuri P, Scita G
Nature communications 2018 Apr 16;9(1):1475
Nature communications 2018 Apr 16;9(1):1475
Rab35 GTPase recruits NDP52 to autophagy targets.
Minowa-Nozawa A, Nozawa T, Okamoto-Furuta K, Kohda H, Nakagawa I
The EMBO journal 2017 Sep 15;36(18):2790-2807
The EMBO journal 2017 Sep 15;36(18):2790-2807
Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.
Takasugi M, Okada R, Takahashi A, Virya Chen D, Watanabe S, Hara E
Nature communications 2017 Jun 6;8:15729
Nature communications 2017 Jun 6;8:15729
Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.
Biesemann A, Gorontzi A, Barr F, Gerke V
The Journal of biological chemistry 2017 Jul 14;292(28):11631-11640
The Journal of biological chemistry 2017 Jul 14;292(28):11631-11640
Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.
Wang HH, Cui Q, Zhang T, Wang ZB, Ouyang YC, Shen W, Ma JY, Schatten H, Sun QY
Histochemistry and cell biology 2016 Jun;145(6):647-57
Histochemistry and cell biology 2016 Jun;145(6):647-57
Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.
Chiu CC, Yeh TH, Lai SC, Weng YH, Huang YC, Cheng YC, Chen RS, Huang YZ, Hung J, Chen CC, Lin WY, Chang HC, Chen YJ, Chen CL, Chen HY, Lin YW, Wu-Chou YH, Wang HL, Lu CS
Oncotarget 2016 Aug 23;7(34):54215-54227
Oncotarget 2016 Aug 23;7(34):54215-54227
Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sheehan P, Zhu M, Beskow A, Vollmer C, Waites CL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 17;36(33):8668-86
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 17;36(33):8668-86
Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.
Dutta D, Donaldson JG
Traffic (Copenhagen, Denmark) 2015 Sep;16(9):994-1009
Traffic (Copenhagen, Denmark) 2015 Sep;16(9):994-1009
Identification of an oncogenic RAB protein.
Wheeler DB, Zoncu R, Root DE, Sabatini DM, Sawyers CL
Science (New York, N.Y.) 2015 Oct 9;350(6257):211-7
Science (New York, N.Y.) 2015 Oct 9;350(6257):211-7
Novel functions for the endocytic regulatory proteins MICAL-L1 and EHD1 in mitosis.
Reinecke JB, Katafiasz D, Naslavsky N, Caplan S
Traffic (Copenhagen, Denmark) 2015 Jan;16(1):48-67
Traffic (Copenhagen, Denmark) 2015 Jan;16(1):48-67
Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.
Miyamoto Y, Yamamori N, Torii T, Tanoue A, Yamauchi J
Molecular biology of the cell 2014 May;25(9):1532-42
Molecular biology of the cell 2014 May;25(9):1532-42
CLIC4 regulates cell adhesion and β1 integrin trafficking.
Argenzio E, Margadant C, Leyton-Puig D, Janssen H, Jalink K, Sonnenberg A, Moolenaar WH
Journal of cell science 2014 Dec 15;127(Pt 24):5189-203
Journal of cell science 2014 Dec 15;127(Pt 24):5189-203
Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.
Zhu Y, Shen T, Liu J, Zheng J, Zhang Y, Xu R, Sun C, Du J, Chen Y, Gu L
Cellular signalling 2013 May;25(5):1075-85
Cellular signalling 2013 May;25(5):1075-85
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.
Frühbeis C, Fröhlich D, Kuo WP, Amphornrat J, Thilemann S, Saab AS, Kirchhoff F, Möbius W, Goebbels S, Nave KA, Schneider A, Simons M, Klugmann M, Trotter J, Krämer-Albers EM
PLoS biology 2013 Jul;11(7):e1001604
PLoS biology 2013 Jul;11(7):e1001604
Syntaxin 16 is a master recruitment factor for cytokinesis.
Neto H, Kaupisch A, Collins LL, Gould GW
Molecular biology of the cell 2013 Dec;24(23):3663-74
Molecular biology of the cell 2013 Dec;24(23):3663-74
TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes.
Davey JR, Humphrey SJ, Junutula JR, Mishra AK, Lambright DG, James DE, Stöckli J
Traffic (Copenhagen, Denmark) 2012 Oct;13(10):1429-41
Traffic (Copenhagen, Denmark) 2012 Oct;13(10):1429-41
MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a.
Rahajeng J, Giridharan SS, Cai B, Naslavsky N, Caplan S
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):82-93
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):82-93
Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35.
Fukuda M, Kobayashi H, Ishibashi K, Ohbayashi N
Cell structure and function 2011;36(2):155-70
Cell structure and function 2011;36(2):155-70
EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.
Imai A, Yoshie S, Ishibashi K, Haga-Tsujimura M, Nashida T, Shimomura H, Fukuda M
The Journal of biological chemistry 2011 Sep 30;286(39):33854-62
The Journal of biological chemistry 2011 Sep 30;286(39):33854-62
Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis.
Egami Y, Fukuda M, Araki N
Journal of cell science 2011 Nov 1;124(Pt 21):3557-67
Journal of cell science 2011 Nov 1;124(Pt 21):3557-67
Androgen-related expression of G-proteins in ovarian cancer.
Sheach LA, Adeney EM, Kucukmetin A, Wilkinson SJ, Fisher AD, Elattar A, Robson CN, Edmondson RJ
British journal of cancer 2009 Aug 4;101(3):498-503
British journal of cancer 2009 Aug 4;101(3):498-503
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 11329-2-AP(RAB35 antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RAB35 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RAB35. This antibody recognizes human,mouse,rat antigen. The RAB35 antibody has been validated for the following applications: ELISA, WB, IP, IF, IHC analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-RAB35 (IP:11329-2-AP, 3ug; Detection:11329-2-AP 1:300) with mouse brain tissue lysate 8000ug.
- Sample type
- tissue
