Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [6]
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Validation data
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- Product number
- NBP2-22119 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal iNOS Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Continuous Immune-Modulatory Effects of Human Olig2+ Precursor Cells Attenuating a Chronic-Active Model of Multiple Sclerosis.
Anti-inflammatory effect of β2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity.
PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function.
NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.
Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice.
Human coronary thrombus formation is associated with degree of plaque disruption and expression of tissue factor and hexokinase II.
Loss of NADPH oxidase-derived superoxide skews macrophage phenotypes to delay type 1 diabetes.
Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome.
Nishri Y, Hampton D, Ben-Shushan E, Fainstein N, Magnani D, Aharonowiz M, Reubinoff BE, Chandran S, Ben-Hur T
Molecular neurobiology 2020 Feb;57(2):1021-1034
Molecular neurobiology 2020 Feb;57(2):1021-1034
Anti-inflammatory effect of β2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity.
Gálvez I, Martín-Cordero L, Hinchado MD, Álvarez-Barrientos A, Ortega E
Brain, behavior, and immunity 2019 Aug;80:564-572
Brain, behavior, and immunity 2019 Aug;80:564-572
PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function.
Pistorius K, Souza PR, De Matteis R, Austin-Williams S, Primdahl KG, Vik A, Mazzacuva F, Colas RA, Marques RM, Hansen TV, Dalli J
Cell chemical biology 2018 Jun 21;25(6):749-760.e9
Cell chemical biology 2018 Jun 21;25(6):749-760.e9
NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.
Bastian C, Zaleski J, Stahon K, Parr B, McCray A, Day J, Brunet S, Baltan S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 11;38(28):6247-6266
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 11;38(28):6247-6266
Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice.
Omlor AJ, Le DD, Schlicker J, Hannig M, Ewen R, Heck S, Herr C, Kraegeloh A, Hein C, Kautenburger R, Kickelbick G, Bals R, Nguyen J, Dinh QT
Small (Weinheim an der Bergstrasse, Germany) 2017 Mar;13(10)
Small (Weinheim an der Bergstrasse, Germany) 2017 Mar;13(10)
Human coronary thrombus formation is associated with degree of plaque disruption and expression of tissue factor and hexokinase II.
Okuyama N, Matsuda S, Yamashita A, Moriguchi-Goto S, Sameshima N, Iwakiri T, Matsuura Y, Sato Y, Asada Y
Circulation journal : official journal of the Japanese Circulation Society 2015;79(11):2430-8
Circulation journal : official journal of the Japanese Circulation Society 2015;79(11):2430-8
Loss of NADPH oxidase-derived superoxide skews macrophage phenotypes to delay type 1 diabetes.
Padgett LE, Burg AR, Lei W, Tse HM
Diabetes 2015 Mar;64(3):937-46
Diabetes 2015 Mar;64(3):937-46
Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome.
Wang Y, Wang W, Zhang Y, Zhao X, Yang D
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2010 Aug;30(4):437-42
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2010 Aug;30(4):437-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - Analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on MCF7 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).