MA5-17139

antibody from Invitrogen Antibodies

Targeting: NOS2 HEP-NOS, iNOS, NOS, NOS2A

Provider product page for MA5-17139

Western blot

ELISA

Antibody data

Antibody Data
Antigen structure
References [8]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [1]
Immunohistochemistry [2]
Flow cytometry [1]
Other assay [2]

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Product number: MA5-17139 - Provider product page
Provider: Invitrogen Antibodies
Product name: iNOS Monoclonal Antibody (4E5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17139 targets NOS2 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17139 immunogen is purified recombinant fragment of human NOS2 expressed in E. Coli. MA5-17139 detects NOS2 which has a predicted molecular weight of approximately 131kDa.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 4E5
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NOS2 protein structure - MA5-17139 shown in red.

Submitted references FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape.

Wu L, Xu Y, Zhao H, Zhou Y, Chen Y, Yang S, Lei J, Zhang J, Wang J, Wu Y, Li Y
Theranostics 2022;12(2):842-858

Local Delivery of Pirfenidone by PLA Implants Modifies Foreign Body Reaction and Prevents Fibrosis.

Fayzullin A, Churbanov S, Ignatieva N, Zakharkina O, Tokarev M, Mudryak D, Khristidis Y, Balyasin M, Kurkov A, Golubeva EN, Aksenova NA, Dyuzheva T, Timashev P, Guller A, Shekhter A
Biomedicines 2021 Jul 21;9(8)

Platelet-Like Fusogenic Liposome-Mediated Targeting Delivery of miR-21 Improves Myocardial Remodeling by Reprogramming Macrophages Post Myocardial Ischemia-Reperfusion Injury.

Tan H, Song Y, Chen J, Zhang N, Wang Q, Li Q, Gao J, Yang H, Dong Z, Weng X, Wang Z, Sun D, Yakufu W, Pang Z, Huang Z, Ge J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Aug;8(15):e2100787

TRPV4 Plays a Role in Matrix Stiffness-Induced Macrophage Polarization.

Dutta B, Goswami R, Rahaman SO
Frontiers in immunology 2020;11:570195

RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis.

Wu L, Zhang X, Zheng L, Zhao H, Yan G, Zhang Q, Zhou Y, Lei J, Zhang J, Wang J, Xin R, Jiang L, Peng J, Chen Q, Lam SM, Shui G, Miao H, Li Y
Cancer immunology research 2020 May;8(5):710-721

Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms.

Sadler R, Cramer JV, Heindl S, Kostidis S, Betz D, Zuurbier KR, Northoff BH, Heijink M, Goldberg MP, Plautz EJ, Roth S, Malik R, Dichgans M, Holdt LM, Benakis C, Giera M, Stowe AM, Liesz A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 29;40(5):1162-1173

S100A9 Regulates MDSCs-Mediated Immune Suppression via the RAGE and TLR4 Signaling Pathways in Colorectal Carcinoma.

Huang M, Wu R, Chen L, Peng Q, Li S, Zhang Y, Zhou L, Duan L
Frontiers in immunology 2019;10:2243

A RIPK3-PGE(2) Circuit Mediates Myeloid-Derived Suppressor Cell-Potentiated Colorectal Carcinogenesis.

Yan G, Zhao H, Zhang Q, Zhou Y, Wu L, Lei J, Wang X, Zhang J, Zhang X, Zheng L, Du G, Xiao W, Tang B, Miao H, Li Y
Cancer research 2018 Oct 1;78(19):5586-5599

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NOS2 using a NOS2 monoclonal antibody (Product # MA5-17139) against a human NOS2 (AA: 997-1058) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NOS2 using a NOS2 monoclonal antibody (Product # MA5-17139) against a human NOS2 (AA: 997-1058) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NOS2 using NOS2 monoclonal antibody (Product # MA5-17139) in Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of iNOS Monoclonal Antibody (4E5) was performed using 70% confluent log phase Hep G2 cells treated with 4 ng/mL of IL-1 beta for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with iNOS Monoclonal Antibody (4E5) (Product # MA5-17139) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 monoclonal antibody (Product # MA5-17139) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NOS2 monoclonal antibody (Product # MA5-17139) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of MCF-7 cells using NOS2 monoclonal antibody (Product # MA5-17139) (green) and negative control (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 TRPV4 regulates matrix stiffness-induced M1 macrophage polarization in vivo as shown by IHC analysis. (A) Skin tissues from WT and TRPV4 KO mice subcutaneously injected with bleomycin or saline were co-stained with CD68 (red) plus iNOS (brown) or CD68 (red) plus Arginase-1 (brown) as indicated. IHC with IgGs was used as a negative control. n = 4 images for each group. Magnification: 20x. (B) Bar graph shows the ratio of iNOS-positive macrophages to Arg-1-positive macrophages from experiment shown in A. n = 5, Student's t -test; *** p < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 10 Semi-quantitative scoring analysis of the expression of immunohistochemical markers in PIC examined by the intensity of staining (see Table 1 for the criteria): ( a ) alpha-SMA, ( b ) iNOS, and ( c ) Arg1. The results are presented as scatterplots, Mean +- SD, * p