AP11772c

antibody from Abcepta

Targeting: NOS2 HEP-NOS, iNOS, NOS, NOS2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP11772c

Provider

Abcepta

Proper citation

Abgent Cat#AP11772c, RRID:AB_10818067

Product name

NOS2A Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.45 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

NOS2A Antibody (Center) (Cat. #AP11772c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOS2A antibody detected the NOS2A protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of NOS2A Antibody (Center)(Cat#AP11772c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

NOS2A Antibody (Center) (Cat. #AP11772c)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOS2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

NOS2A Antibody (Center) (Cat. #AP11772c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50