TA336918

antibody from OriGene

Targeting: NOS2 HEP-NOS, iNOS, NOS, NOS2A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA336918

Provider

OriGene

Product name

Mouse Monoclonal iNOS Antibody (4E5)

Antibody type

Monoclonal

Description

Mouse Monoclonal iNOS Antibody (4E5)

Host

Mouse

Conjugate

Unconjugated

Epitope

NOS2

Isotype

IgG

Antibody clone number

400000

Vial size

100 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: iNOS Antibody (4E5) [TA336918] - Western blot analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: iNOS Antibody (4E5) [TA336918] - Western blot analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ELISA: iNOS Antibody (4E5) [TA336918] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Validation comment

ELISA

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [TA336918] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [TA336918] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometry: iNOS Antibody (4E5) [TA336918] - Flow cytometric analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).

Validation comment

FC